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TAPSO CAS:68399-81-5 Utu Kaihanga
Ko te TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) he pūhui zwitterionic e whakamahia whānuitia ana i roto i ngā rangahau koiora me te matū koiora. He kaihoko pūhui whai hua me te pKa e tata ana ki te pH ā-tinana, e pai ai mō te pupuri i te pH pumau i roto i ngā whakamātautau koiora. He maha ngā wā ka whakamahia te TAPSO i roto i te purenga pūmua, ngā whakamātautau whākōkī, te ahurea pūtau, me ngā tono electrophoresis. Nā tōna wairewatanga wai teitei me te iti o te pokanoa ki ngā tukanga koiora, he mea rongonui i roto i te hapori pūtaiao. E mōhiotia ana te TAPSO mō tōna pānga iti ki te mahi whākōkī, ā, he maha ngā wā ka whakamahia hei whakakapi mō ētahi atu kaihoko pūhui pērā i te Tris, i ngā pūhui phosphate rānei.
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6-Methyl-2-pyridinyl2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside CAS:149263-94-5
Ko te 6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside, e mōhiotia ana ko te N-acetylglucosamine thiolactone, he matū he hanganga motuhake kei roto he mowhiti pyridine, he rōpū acetylamino, me te wāhanga thiolactone.
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Konutai Glycocholate CAS:863-57-0
He matū tote au te konutai glycocholate e āwhina ana i te nakunaku me te mimiti o ngā ngako kai. Ka hangaia mai i te glycine me te waikawa cholic, ā, he mahi nui tana ki te wawahi i ngā ngako ki ngā pata iti ake, ka whakanui ake i te horahanga o te mata kia pai ai te nakunaku. He tino wairewa te konutai glycocholate i roto i te wai, ā, e whakamahia ana i roto i ngā hanganga rongoā hei whakarei ake i te wairewatanga me te wātea o ngā rongoā kāore e tino wairewa. Hei tāpiri, e whakamahia ana i roto i ngā rangahau me ngā taiwhanga hei tautoko i te tipu o ētahi huakita me ngā momo pūtau..
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TAPS Tote konutai CAS:70331-82-7
Ko te tote konutai TAPS, e mōhiotia ana ko te tote konutai N-(Tris(hydroxymethyl)methyl)-2-aminoethanesulfonic acid, he mea whakapūmau i ngā āhuatanga maha, he mea whakahaere pH hoki. He mea whakamahi noa i roto i ngā rangahau koiora me te matū, ngā rongoā, me ngā tātaritanga. Ko te TAPS-Na he matū zwitterionic e āwhina ana ki te pupuri i te pH pumau i roto i ngā otinga, e pai ai mō ngā momo āhuatanga whakamātautau. Kei roto i ōna whakamahinga ko te ahurea pūtau, te rangahau pūmua, te electrophoresis, te hanga matū, me ngā hanganga rongoā. Ko te TAPS-Na he momo TAPS wairewa, pumau hoki, he ngāwari ki te whakahaere me te whakamahi i roto i ngā otinga wai.
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NBD pūhaumāota CAS:10199-89-0
Ko te NBD chloride, e mōhiotia ana ko te 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, he tae kanapa e whakamahia whānuitia ana i roto i ngā mahi matū me ngā mahi koiora. Nō te akomanga nitrobenzoxadiazole (NBD) derivatives, e whakaatu ana i ngā āhuatanga kanapa kaha.
Ko te NBD chloride te āhuatanga o tōna kaha ki te hanga hononga covalent me ngā nucleophiles, pērā i ngā amines tuatahi, ngā thiols, me ngā rōpū hydroxyl. Mā tēnei āhuatanga ka taea te whakamahi hei tohu mō ngā biomolecules, tae atu ki ngā pūmua, ngā peptides, ngā waikawa nucleic, me ētahi atu ngota iti.
Ina tauhohe te pūhaumāota NBD ki tētahi nucleophile, ka pāngia e te tauhohenga whakakapinga, ka hua ake he hua pumau kua tohua ki te NBD. Ka whakaatuhia e tēnei hua kua tohua he tukunga fluorescence kaha, ā, he mea whai hua mō ngā tikanga kimi me te whakaahua.
Ka taea te whakaoho i te tukunga hihi-ā-rangi o ngā ngota i tapaina ki te NBD me te mārama ultraviolet (UV) i te 470 nm pea, ā, ko te mārama e tukuna ana ka kitea i te 530 nm pea. Ka taka tēnei roangaru tukunga ki roto i te rohe matomato o te whānuitanga e kitea ana, he iti noa iho te pokanoa ki te kimi mai i ngā tauira koiora.
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Proteinase K CAS:39450-01-6 Utu a te Kaihanga
Ko te Proteinase K he whākōkī serine protease tino kaha e taea ai te whakakorikori i te waikura o ngā here peptide i roto i ngā pūmua. I ahu mai i te harore Engyodontium album, ā, e mōhiotia ana mō tōna whānuitanga o te motuhaketanga o te substrate. Ka taea e te Proteinase K te tapahi pai i ngā here peptide i te taha C-terminal o ngā waikawa amino hydrophobic, aliphatic, aromatic rānei, e whai hua ai i roto i ngā tono koiora ngota me te matū koiora..
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N,N-Dimethyl-p-phenylenediamine dihydrochloride CAS:536-46-9
Ko te N,N-Dimethyl-p-phenylenediamine dihydrochloride he matū e whakamahia whānuitia ana i roto i ngā mahi pūtaiao me ngā mahi ahumahi. He paura tioata mā ki te mā-parauri, ko tōna tauira ngota ko C8H14N2·2HCl.
He whānuitia ngā whakamahinga o tēnei matū nā ōna āhuatanga me tōna kaha tauhohenga. He maha ngā wā ka whakamahia hei matū tātari mō te whakatau i ngā peroxides me te takawaenga tae i roto i te hanga i ngā momo tae me ngā tae.
I roto i te mara o te matū tātari, he maha ngā wā ka whakamahia te N,N-Dimethyl-p-phenylenediamine dihydrochloride hei tohu tae mō te whakatau i ngā momo waikura, pērā i te hauwai peroxide, i ngā peroxide rānei i roto i ngā tauira. Ka rerekē te tae o te matū i te wā e tauhohe ana ki ēnei matū waikura, ā, ka taea te ine i ō rātou rahinga.
Hei tāpiritanga, ka kitea anō hoki tēnei matū i roto i te hanganga o ngā tae makawe, hei kaiwhakawhanake tae. Nā tōna kaha ki te waihanga i ngā tae kanapa, pumau hoki, ka rongonui ai i roto i te umanga whakapaipai.
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Tote disodium phosphate Creatine CAS:922-32-7
Ko te tote disodium phosphate creatine he matū kei roto ko te creatine, he waikawa amino tūturu e honoa ana ki ngā rōpū phosphate me ngā katote konutai. He mahi nui tāna ki te whakarato pūngao mō te whakaheke uaua, ā, e whakamahia whānuitia ana hei tāpiringa kai mā ngā kaitākaro me ngā kaihanga tinana.
Ina kainga, ka hurihia te tote creatine phosphate disodium hei creatine phosphate i roto i te tinana, ka rongoatia ki roto i ngā uaua. I ngā mahi kaha, pērā i te hiki taumaha, te oma tere rānei, ka mahi te creatine phosphate hei puna hononga phosphate kaha-teitei e wātea ana, e āhei ai te whakakī tere i te adenosine triphosphate (ATP).
Ko te ATP te pūngao matua e whakamahia ana e ngā uaua, ā, ka tere te heke o ōna taumata i te wā e kaha ana te whakakori tinana. Mā te tāpiri atu ki te creatine phosphate, ka taea e ngā kaitākaro te whakanui ake i te wātea o te ATP, ka pai ake te mahi a ngā uaua, ka nui ake te kaha, ka iti ake hoki te ngenge.
Hei tāpiritanga, tera pea ētahi atu painga pea o te tote creatine phosphate disodium, tae atu ki te whakanui ake i te papatipu uaua, te whakaora ake, me te whakapai ake i te mahi hinengaro. I te nuinga o te wā ka tangohia mā te waha i roto i te ahua paura, i te ahua papa rānei, hei tāpiringa takitahi, hei wāhanga rānei o te tikanga tāpiringa i mua i te whakangungu, i muri rānei i te whakangungu.
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2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridiniuM-9-carboxylate 4′-NHS Ester CAS:194357-64-7
He matū matatini te 2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4′-NHS ester. Kei roto he rōpū sulfopropylacridinium me he rōpū mahi carboxylate ester. Ko te aroaro o te wāhanga ester e tohu ana he tauhohe, ā, ka taea te whakamahi hei tapanga, hei kaiwhakarerekē rānei mō ngā koiora ngota.
E kī ana te rōpū sulfopropylacridinium o te matū he pea he tono pea mō ngā whakamātautau e hangai ana ki te fluorescence, ka taea te whakamahi hei tirotiro fluorescence, hei tae rānei mō te kimi me te tātari i ngā koiora ngota. Tērā pea he hiranga anō hoki mō ngā rangahau mō ngā tukanga pūtau, pērā i te tohu konupūmā ā-roto.
Ko te whakaurunga o te rōpū ester NHS e tohu ana ka taea e ia te tauhohe ki ngā amine tuatahi, pērā i ngā mea e kitea ana i roto i ngā pūmua, i ngā peptide rānei, hei hanga hononga amide pumau. Nā tēnei tauhohenga ka whai hua mō ngā tono bioconjugation, ka taea te whakamahi hei tapanga, hei whakarerekē rānei i ngā biomolecules me ētahi atu ngota mahi, pērā i ngā fluorophores, ngā tohu rānei.
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ABEI CAS:66612-29-1 Utu Kaihanga
Ko te N-(4-Aminobutyl)-N-ethylisoluminol, e mōhiotia ana ko ABEI, he matū kanapa e whakamahia ana hei whakamātautau chemiluminescent i roto i ngā tono tātaritanga koiora. Nō te akomanga o ngā matū whakaputa isoluminol, ā, e whakamahia whānuitia ana i roto i ngā whakamātautau ārai mate me ngā tikanga kimi DNA/RNA.
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Z-GLY-PRO-PNA CAS:65022-15-3
Ko te Z-GLY-PRO-PNA he matū i ahu mai i te peptide nucleic acid (PNA) me te raupapa Z-Gly-Pro. He mea whakamahi noa tēnei matū i roto i ngā rangahau me ngā tātaritanga nā tōna kaha ki te herea motuhake ki ngā raupapa waikawa nucleic tāpiri. Mā te tāpiri o te rōpū-Z ki te pito-N ka taea te whai hua me te tuku ki ngā whāinga ngota e hiahiatia ana. Mā tōna huinga ahurei o ngā āhuatanga peptide me te waikawa nucleic, he nui te pūmanawa o te Z-GLY-PRO-PNA i roto i ngā tono maha, tae atu ki te rongoā ira, te tātaritanga ngota, me te whakawhanaketanga rongoā..
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TRIS-Acetate CAS:6850-28-8 Utu Kaihanga
Ko te TRIS-Acetate he pūhui e whakamahia whānuitia ana i roto i ngā whakamātautau koiora me te matū koiora. He huinga o te turanga Tris me te waikawa acetic, ka hua ake he otinga pH-pūmau e whakamahia ana hei whakahaere me te pupuri i te awhe pH e hiahiatia ana mō ngā momo tono. He tino whai hua te TRIS-Acetate i roto i ngā rangahau DNA me te RNA, nā te mea e whakarato ana i te taiao e tika ana mō ngā mahi whākōkī, te electrophoresis, me te gel electrophoresis. Ka āwhina i te pupuri i te pumau me te pono o ngā waikawa karihi i roto i ngā momo tikanga, pērā i te raupapatanga DNA, te tauhohenga mekameka polymerase (PCR), me te agarose gel electrophoresis. Haunga te rangahau waikawa karihi, ka whakamahia hoki te TRIS-Acetate i roto i ngā tikanga wehewehe me te purenga pūmua, te tango pūmua kiriuhi, me ngā whakamātautau ahurea pūtau. Nā tōna kaha pūhui maha e whai hua ai i roto i ngā mara maha o te rangahau pūtaiao, e whakarite ana i ngā āhuatanga tino pai mō ngā tauhohenga koiora me te pupuri i te pumau o ngā whākōkī me ngā pūmua.
