TRIS-Acetate CAS:6850-28-8 Utu Kaihanga

Ko te Tris-acetate (TRIS-Acetate) he mea e whakamahia whānuitia ana i roto i ngā whakamātautau koiora me te matū koiora. Kei roto ko te huinga o te tris(hydroxymethyl)aminomethane (Tris) me te waikawa acetic, e mahi ana hei kaiwhakahaere pH me te whakaū. Ko te pH o te TRIS-Acetate buffer kei waenganui i te 7.4 ki te 8.4.
Ko te pānga matua o te TRIS-Acetate he pupuri i te pH pumau, he mea nui mō ngā tauhohenga koiora me te matū koiora maha. Ka mahi hei ārai mā te whakaiti i ngā huringa nui o te pH ka puta mai i ngā waikawa, i ngā kawa rānei i tāpirihia i roto i ngā tukanga whakamātautau.
He maha ngā whakamahinga o te TRIS-Acetate i roto i te koiora ngota, te matū koiora, me te hangarau koiora:
Te Whakapūtanga Hiko o te DNA me te RNA: He mea whakamahi noa te TRIS-Acetate hei pūmau i roto i te whakapūtanga hiko o te agarose me te polyacrylamide gel. Ka whakaratohia he taiao pH pumau i te wā e wehea ana ngā kongakonga DNA me te RNA i runga i tō rātou rahi.
Tātari Pūmua: Ka whakamahia ngā arai TRIS-Acetate mō te electrophoresis pūmua, pērā i te SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Mā tēnei ka pumau te pūmua me te wehenga i te wā o te tukanga.
Ngā Tauhohenga Whākōkī: He maha ngā whakamahinga o ngā ārai TRIS-Acetate i roto i ngā whakamātautau me ngā rangahau whākōkī. Ka whakaratohia te awhe pH tino pai mō ngā tauhohenga whākōkī, ā, ka āwhina ki te pupuri i te mahi whākōkī.
Ahurea Pūtau me te Kiko: Ka whakamahia ngā ārai TRIS-Acetate i roto i ngā pāpāho ahurea pūtau hei pupuri i te pH tika mō te tipu me te horapa o ngā pūtau. Ka āwhina ki te pupuri i ngā āhuatanga ā-tinana e hiahiatia ana mō te oranga tonutanga o ngā pūtau.