NBD chloride CAS:10199-89-0 Whakaahua Matua

NBD pūhaumāota CAS:10199-89-0

Ko te NBD chloride, e mōhiotia ana ko te 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, he tae kanapa e whakamahia whānuitia ana i roto i ngā mahi matū me ngā mahi koiora. Nō te akomanga nitrobenzoxadiazole (NBD) derivatives, e whakaatu ana i ngā āhuatanga kanapa kaha.

Ko te NBD chloride te āhuatanga o tōna kaha ki te hanga hononga covalent me ngā nucleophiles, pērā i ngā amines tuatahi, ngā thiols, me ngā rōpū hydroxyl. Mā tēnei āhuatanga ka taea te whakamahi hei tohu mō ngā biomolecules, tae atu ki ngā pūmua, ngā peptides, ngā waikawa nucleic, me ētahi atu ngota iti.

Ina tauhohe te pūhaumāota NBD ki tētahi nucleophile, ka pāngia e te tauhohenga whakakapinga, ka hua ake he hua pumau kua tohua ki te NBD. Ka whakaatuhia e tēnei hua kua tohua he tukunga fluorescence kaha, ā, he mea whai hua mō ngā tikanga kimi me te whakaahua.

Ka taea te whakaoho i te tukunga hihi-ā-rangi o ngā ngota i tapaina ki te NBD me te mārama ultraviolet (UV) i te 470 nm pea, ā, ko te mārama e tukuna ana ka kitea i te 530 nm pea. Ka taka tēnei roangaru tukunga ki roto i te rohe matomato o te whānuitanga e kitea ana, he iti noa iho te pokanoa ki te kimi mai i ngā tauira koiora.

Tonohia, Tonoa rānei he Tāpaetanga

Taipitopito Hua

Ngā Tohu Hua

Te Whakamahinga me te Pānga

Te tapanga hononga-konehe: Ka taea e te pūhaumāota NBD te hanga hononga hononga-konehe me ngā rōpū nucleophilic i roto i ngā ngota koiora, pērā i ngā amine tuatahi, ngā thiol, me ngā rōpū hydroxyl. Mā tēnei ka taea te tapanga motuhake o ēnei ngota koiora mō ngā kaupapa kimi me te whakaahua.

Te whakarei ake i te hihi kanapa: Ina tauhohe te pūhaumāota NBD ki ngā nucleophiles, ka puta he hua hihi kanapa kua tohua ki te NBD. Ka whakaatuhia e tēnei hua kua tohua he tukunga hihi kanapa kaha, he mea whai hua mō te tirohanga me te aroturuki i ngā ngota ngota kua tohua.

Te aro me te motuhaketanga: He tino aro te tukunga hihi o ngā hua kua tohua ki te NBD, e āhei ai te kite i te iti o ngā ngota ngota kua tohua. Hei tāpiri, ko te hanganga hononga honohono i waenga i te NBD chloride me ngā nucleophiles e whakarato ana i te motuhaketanga, nā te mea ka whakarite i te piri pumau me te motuhake o te tae ki te koiora koiora e whaihia ana.

Te tapanga ira koiora: Ka taea te whakamahi i te pūhaumāota NBD hei tapanga i ngā momo ira koiora, tae atu ki ngā pūmua, ngā peptide, ngā waikawa karihi, me ētahi atu ira iti. Mā tēnei ka taea e ngā kairangahau te rangahau i te wāhi, te hihiri, me ngā taunekeneke o ēnei ira koiora i roto i ngā pūnaha koiora.

Mā te karuārai hihi-ā-rangi: Ka taea te tiro i ngā ngota koiora kua tohua ki te NBD mā te whakamahi i te karuārai hihi-ā-rangi. Mā tēnei tikanga ka taea te tirotiro i ngā ngota kua tohua ki te taumata pūtau, ki te taumata pūtau-iti rānei, ka puta he mōhiohio whai hua mō tō rātou tohatoha me te whanonga.

Te ine rere o te pūtau: Ka taea te tātari me te ine i ngā ngota koiora kua tohua ki te NBD mā te whakamahi i te ine rere o te pūtau. Mā tēnei tikanga ka taea te tātari tere o ngā pūtau, ngā matūriki rānei kua tohua, e whakarato ana i ngā mōhiohio e pā ana ki ngā taumata whakaaturanga, ki ngā āhuatanga mata rānei o ngā ngota koiora kua tohua.

Te tapanga me te aroturuki i te pūmua: Ka taea te whakamahi i te pūhaumāota NBD hei tapanga i ngā toenga waikawa amino motuhake, i ngā rōpū mahi rānei i roto i ngā pūmua. Mā tēnei ka taea e ngā kairangahau te ako i te hanganga pūmua, te takai, te hihiri, me ngā taunekeneke i te wā tonu.

Te Tākai Hua:

Ngā Mōhiotanga Tāpiri:

Te hanganga	C6H2ClN3O3
Whakamātautau	99%
Āhua	Paura kōwhai
Nama CAS	10199-89-0
Te tākai	Iti, nui hoki
Te Ora o te Wāhi	2 tau
Rokiroki	Penapena ki te wāhi hauhautanga, maroke hoki
Tiwhikete	ISO.