Phenylgalactoside CAS:2818-58-8

Te awenga ki te ngohe o te whaakiki: Ka whakamahia te Phenylgalactoside ki te ine i te mahi o te enzyme β-galactosidase.A, no te phenylgalactoside te hauwai e te β-galactosidase, ka tukuna e ia te p-nitrophenol.Ko te kohikohinga o te p-nitrophenol ka taea te ine ine, e whakaatu ana i nga whakaaro mo te mahi o te β-galactosidase.Ka whakamahia tenei paanga i roto i nga tono penei i te whakamatautau i te enzyme me nga punaha tirotiro.

Te tātari ira ira: Ka whakamahia te Phenylgalactoside hei tïpako i roto i nga whakamatautau koiora rapoi ki te ako i te whakaaturanga ira.Ko te ira lacZ, e whakawaehere ana i te β-galactosidase, ka honoa ki nga raupapa ture o etahi atu ira e whai paanga ana.Ko te whakapuakitanga o te ira lacZ me te whakamaaramatanga o te phenylgalactoside na te β-galactosidase ka taea te whakaatu i te tauira korero me te taumata o te ira whainga e akohia ana.

Nga punaha tirotiro: Ko te Phenylgalactoside tetahi waahanga nui o nga punaha tirotiro e whakamahi ana i te mahi β-galactosidase.Ko tetahi tauira e mohiotia nuitia ana ko te tikanga tirotiro puru-ma, e whakamahia ana hei tautuhi i nga pūtau whakahiato, kua huri ranei i roto i nga whakamatautau koiora ngota.Ko nga koroni kua angitu te tango i te DNA recombinant, i uru ranei ki te whakakotahitanga ira ka whakaatu i te β-galactosidase, ka arai ki te hauwai o te phenylgalactoside me te hanganga o te tae kikorangi.

Te purenga pūmua: I etahi wa, ka taea te whakamahi i te phenylgalactoside hei herenga mo te chromatography affinity ki te pure i nga pūmua e herea ana, e whakahohehia ana ranei e te β-galactosidase.Ko te pūmua o te hiahia he tohu hononga, he tohu whakakotahitanga kei roto he rohe here-β-galactosidase.Ma te whakawhiti i te ranunga pūmua i roto i te tīwae me te phenylgalactoside kaore i nekehia, ka taea te pupuri i te pūmua e hiahiatia ana ka kohia i muri mai.