Phenylgalactoside CAS:2818-58-8

Pānga ki te mahi a te whākōkī: He mea noa te whakamahi i te Phenylgalactoside hei ine i te mahi a te whākōkī β-galactosidase. Ina whakahāngaihia te phenylgalactoside e te β-galactosidase, ka tukuna te p-nitrophenol. Ka taea te ine i te kohikohinga o te p-nitrophenol mā te ine ine, ka hoatu he māramatanga ki te mahi a te β-galactosidase. Ka whakamahia tēnei pānga i roto i ngā tono pēnei i ngā whakamātautau whākōkī me ngā pūnaha tirotiro.

Tātaritanga Whakapuakitanga Ira: He maha ngā wā ka whakamahia te Phenylgalactoside hei tūāpapa i roto i ngā whakamātautau koiora ngota hei ako i te whakapuakanga ira. Ko te ira lacZ, e whakakōwae ana i te β-galactosidase, ka honoa ki ngā raupapatanga whakahaere o ētahi atu ira e hiahiatia ana. Ko te whakapuakanga o te ira lacZ me te waikura o te phenylgalactoside e te β-galactosidase ka taea te tohu i te tauira whakapuakanga me te taumata o te ira ūnga e akohia ana.

Ngā pūnaha tirotiro: He wāhanga nui te Phenylgalactoside o ngā pūnaha tirotiro e whakamahi ana i te mahi β-galactosidase. Ko tētahi tauira e mōhiotia whānuitia ana ko te tikanga tirotiro kikorangi-mā, e whakamahia ana hei tautuhi i ngā pūtau recombinant, ngā pūtau kua hurihia rānei i roto i ngā whakamātautau koiora ngota. Ko ngā koroni kua angitu te tango i te DNA recombinant, kua uru rānei ki te recombination ira ka whakaatu i te β-galactosidase, ka arahi ki te hydrolysis o te phenylgalactoside me te hanganga o te tae kikorangi.

Te purenga pūmua: I ētahi wā, ka taea te whakamahi i te phenylgalactoside hei ligand mō te chromatography hononga hei pure i ngā pūmua e herea motuhake ana ki te β-galactosidase, e whakahohehia ana rānei. Tērā pea he tohu hononga, he tohu whakakotahitanga rānei kei roto i te pūmua e hiahiatia ana he rohe herenga β-galactosidase. Mā te tuku i te ranunga pūmua mā roto i te pou me te phenylgalactoside kua whakapūmautia, ka taea te pupuri motuhake i te pūmua e hiahiatia ana, ā, muri iho ka tangohia.