4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Ko te pānga o te 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) he mahi hei tūāpapa mō te whākōkī beta-glucosidase. Ka tapahia e tēnei whākōkī te here glucosidic o te MUG, ka puta te tukunga o te 4-Methylumbelliferone (4-MU). Ko te whakamahinga o te MUG kei roto i te mara o te moroiti, inā koa mō te kimi me te tautuhi i ngā huakita e whakaputa ana i te beta-glucosidase. He mea whakamahi noa te MUG i roto i te kimi i te Escherichia coli (E. coli) i roto i ngā tauira wai me ngā tauira kai. Kei a E. coli te whākōkī beta-glucosidase, ka taea te whakawhaiti i te MUG me te whakaputa i tētahi tohu hihi kanapa i te aroaro o te mārama ultraviolet (UV). Mā te āhuatanga hihi kanapa o te 4-MU ka ngāwari te kimi me te ine. Ina whakawhaitihia te tūāpapa MUG, ka tukuna e te 4-MU he hihi kanapa kikorangi, ka āwhina i te tautuhi i ngā huakita he mahi beta-glucosidase. He tikanga tēnei e whakamahia whānuitia ana i roto i te aroturukitanga taiao me te whakamātautau haumaru kai, nā te mea he huarahi tere, he tairongo hoki ki te kite i te poke o ngā huakita. Haunga tōna whakamahinga i roto i te koiora moroiti, ka taea hoki te whakamahi i te MUG ki te ako i te mahi me te aukati i te beta-glucosidase i roto i te matū koiora me te rangahau koiora ngota. Mā tōna fluorescence ka taea te ine i te kaha o te whākōkī, ā, ka taea te whakamahi hei tirotiro mō ngā aukati pea, ngā kaiwhakaoho rānei o te mahi beta-glucosidase. I te nuinga o te wā, he matū maha ngā mahi a te MUG e kitea ana te whakamahinga whānui i roto i ngā mara o te koiora moroiti, te matū koiora, me te koiora ngota mō te kite i te mahi beta-glucosidase me te tautuhi i ngā huakita e whakaputa ana i tēnei whākōkī.