ONPG CAS:369-07-3 Utu Kaihanga

Ko te pānga o te ONPG hei matūriki ka tapahia e te whākōkī β-galactosidase, ka hua ake he hua kōwhai, ko te o-nitrophenol. Ka taea te ine i tēnei huringa tae mā te ine irahiko, ka taea ai te ine i te mahi a te β-galactosidase. Ko te whakamahinga o te ONPG ko te aromatawai i te whakaaturanga ira i roto i te koiora ngota me te rangahau moroiti. He mea whakamahi noa hei ine i te mahi β-galactosidase hei kairīpoata mō ngā rangahau whakaaturanga ira, inā koa i roto i ngā huakita pērā i te E. coli. Ko te ira lacZ, e whakakōrero ana i te β-galactosidase, he mea whakamahi pinepine hei tohu mō te tātari whakaaturanga ira, nā te mea ka taea te whakaoho i tōna whakaaturanga e ngā āhuatanga motuhake, e whakahaeretia rānei e ngā kaiwhakatairanga motuhake. Ka whakaratohia e te whakamātautau ONPG he tikanga watea me te pono hei aromatawai i te taumata o te whakaaturanga ira mā te ine i te mahi a te β-galactosidase. He whānuitia te whakamahinga o tēnei whakamātautau i roto i ngā tono maha, pērā i te ako i te mahi kaiwhakatairanga, te whakahaere ira, me ngā taunekeneke pūmua-pūmua. Hei tāpiri, ka taea te whakamahi hei whakatau i te kinetics whākōkī me te aromatawai i ngā pānga o ngā whakarerekētanga, ngā maimoatanga rānei ki te mahi whākōkī.