Nitrotetrazolium Pūhaumāota kikorangi CAS: 298-83-9

Ko te Nitrotetrazolium Blue Chloride (NBT) he tohu redox e whakamahia whānuitia ana i roto i ngā whakamātautau koiora me te matū koiora. He paura kōwhai mārama ka huri ki te kikorangi ina whakaitihia, ā, he mea whai hua mō te kite i te aroaro o ētahi whākōkī me te mahi pākia.

Ko te pānga matua o te NBT ko te hanganga o tētahi matū formazan kikorangi ina whakaitihia e ētahi whākōkī. Mā tēnei huringa tae ka taea te kite i te mahi whākōkī mā te tirohanga, mā te ine-ira rānei.

He maha ngā whakamahinga o te NBT i roto i te rangahau me te tātaritanga. Anei ētahi o ōna whakamahinga matua:

Ngā whakamātautau ngohe whākōkī: Ka taea te whakamahi i te NBT hei ine i te ngohe o ngā dehydrogenases, e whai wāhi ana ki ngā tukanga pēnei i te manawa pūtau me te pākia o te pūtau. Mā te aroturuki i te whakahekenga o te NBT ki te formazan, ka taea e ngā kairangahau te aromatawai i te ngohe o ēnei whākōkī.

Aromatawai i te mahi a ngā pūtau ārai mate: E whakamahia whānuitia ana te NBT i roto i te whakamātautau whakaiti NBT hei aromatawai i te kaha o te pahūtanga manawa o ngā pūtau ārai mate, inā koa ko ngā pūtau whakapūpū. Ka ine te whakamātautau i te kaha o ēnei pūtau ki te whakaputa i ngā momo hāora tauhohe, ka taea te whakaiti i te NBT me te hanga i tētahi putunga kikorangi.

Rangahau moroiti: E whakamahia ana te NBT i roto i te moroiti hei ako i te pākia o ngā moroiti me te aromatawai i te mahi a ngā whākōkī motuhake. Hei tauira, kua whakamahia hei kimi i ngā reductase nitrate huakita, i ngā huakita hanga formazan rānei.

Ngā rangahau oranga pūtau: Mā te whakaiti i te NBT ka taea e ngā kairangahau te aromatawai i te ngohe pākia me te oranga o ngā pūtau. Mā te ine i te kaha o te hua formazan kikorangi, ka taea te whakatau i te maha o ngā pūtau ora i roto i tētahi tauira kua hoatu.