Disodium 4-nitrophenylphosphate CAS:4264-83-9

Ko te pānga matua me te whakamahinga o te disodium 4-nitrophenylphosphate ko tōna whakamahinga hei matū chromogenic mō ngā whakamātautau whākōkī alkaline phosphatase. Ina whakatōkia tēnei matū ki te alkaline phosphatase, ka tapahia e te whākōkī te rōpū phosphate mai i te disodium 4-nitrophenylphosphate, ka hua ake te hanganga o te 4-nitrophenol.

Ko te 4-nitrophenol i puta mai he matū tae kōwhai ka taea te kite mā te ine ine i te 405 nm. Ka whakamahia tēnei panoni tae hei ine i te mahi a te alkaline phosphatase i roto i ngā tauira, pērā i ngā wai koiora, i ngā pūtau lysates rānei.

Mā te whakamahi i te disodium 4-nitrophenylphosphate i roto i ngā whakamātautau whākōkī ka taea te ine i te mahi a te alkaline phosphatase. Kei roto tēnei whākōkī i ngā tukanga koiora maha, ā, e whakamahia whānuitia ana hei tohu mō te rerekētanga o te kiko, te mahi ate, me te pākia o te wheua.

Hei tāpiritanga, ka taea te whakamahi i te disodium 4-nitrophenylphosphate hei tūāpapa mō ētahi atu phosphatases, pērā i te acid phosphatase, i te protein phosphatases rānei, i runga i te whakatakotoranga whakamātautau me te whāinga rangahau.