Streptavidin mai i Streptomyces avidinii CAS:9013-20-1

Hononga here: He tino kaha te hononga o Streptavidin ki te biotin, ā, he taputapu pai rawa atu tēnei mō te hopu me te kite i ngā ngota ira kua whakapūmautia ki te biotinylation.

Te purenga pūmua: He maha ngā wā ka whakamahia te streptavidin hei taputapu purenga i roto i te hangarau koiora. Ka taea te tāpiri i ngā tohu, ngā ngota rānei kua whakawhaitihia ki ngā pītiti streptavidin, kia taea ai te wehe me te purenga o ngā pūmua motuhake mai i ngā ranunga matatini.

Ngā whakamātautau kitenga: He mea whakamahi noa te Streptavidin i roto i ngā whakamātautau ārai mate, pērā i ngā whakamātautau immunosorbent enzyme-linked (ELISA), ka taea e ia te here i ngā paturopi kitenga biotinylated, i ngā antigen rānei. Mā tēnei ka taea te kite tairongo me te motuhake i ngā momo pūmua, i ngā tohu koiora rānei.

Te tuku rongoā kua arotahihia: Ka taea te whakamahi i te Streptavidin i roto i ngā pūnaha tuku rongoā mā te hono atu ki ngā kaihoko rongoā, ki ngā matūriki nano rānei. Kātahi ka taea te tāpiri i ngā ngota arotahi kua whakawhaitihia ki te streptavidin, kia pai ake ai te tuku kua arotahihia ki ngā pūtau, ki ngā kiko rānei.

Ngā tikanga koiora ngota: Ka whakamahia te Streptavidin i roto i ngā tikanga koiora ngota, pērā i ngā whakamātautau whakaranu waikawa karihi me te tauhohenga mekameka polymerase (PCR). Ka taea te whakamahi i ngā probes biotinylated, i ngā primers rānei hei kimi, hei whakanui rānei i ngā waikawa karihi motuhake, ā, ka whakamahia te streptavidin hei tiro, hei hopu rānei i ngā hua biotinylated.

Whakaahua: He maha ngā wā ka whakamahia a Streptavidin hei taputapu i roto i te karuārai hihi me ngā tikanga whakaahua. Mā te whakakotahi i ngā tae hihi me te streptavidin, ka taea te tapanga me te tiro i ngā ūnga motuhake me te tino aro me te taumira.

Tātaritanga: He whānuitia te whakamahinga o ngā whakamātautau streptavidin i roto i ngā tātaritanga haumanu hei kimi i ngā mate, i ngā huakita rānei. Ka taea e ngā whakamātautau biotinylated te kimi i ngā waikawa karihi, i ngā antigen rānei, ā, ka taea te tātaritanga me te aroturuki i ngā āhuatanga rerekē.