Pūmua K mō te pukaemi tritirachium CAS:39450-01-6

Ko te Proteinase K he whākōkī i tangohia mai i te harore Tritirachium album. He protease serine whānui-motuhake tēnei, arā, ka taea e ia te tapahi i ngā hononga peptide i roto i te whānuitanga o ngā pūmua. E mōhiotia ana te Proteinase K mō tōna kaha ki te wawahi pai i ngā pūmua, ā, he taputapu whai hua tēnei mō ngā momo whakamahinga.

Ko tētahi o ngā whakamahinga matua o te Proteinase K ko te rangahau koiora ngota. He mea whakamahi noa mō ngā tikanga tango DNA me te RNA hei tango i ngā pūmua ka taea te whakararuraru i te tātaritanga o muri. Ka āwhina a Proteinase K ki te wawahi i ngā pūtau me te whakaiti i ngā pūmua pūtau, ka taea ai te wehe i ngā waikawa karihi kounga teitei.

E whakamahia whānuitia ana hoki te Proteinase K i roto i ngā tātaritanga pūmua me ngā rangahau āhuatanga. Ka taea te whakamahi mō te nakunaku pūmua, ka taea ai te whakaputa i ngā kongakonga peptide ka taea te tātari mā roto i ngā tikanga pēnei i te ine papatipu. Hei tāpiri, he pai te Proteinase K mō te raupapatanga pūmua me te tautuhi i te tūranga o ngā waikawa amino i roto i te mekameka polypeptide.

Ko tētahi atu whakamahinga nui o te Proteinase K ko te horoi i ngā mata. He mea whakamahi noa i roto i ngā taiwhanga koiora ngota hei tango i ngā waikawa karihi, ngā pūmua, me ētahi atu poke mai i ngā taputapu taiwhanga me ngā mata. He tino pumau te Proteinase K, ā, he kaha hoki i raro i te whānuitanga o ngā āhuatanga, tae atu ki ngā pāmahana teitei me ngā taumata pH rerekē.