P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Te kimi i te mahi a te beta-galactosidase: E whakamahia whānuitia ana te PNPG i roto i ngā whakamātautau hei ine i te mahi a te beta-galactosidase, he whākōkī e whakateretere ana i te waikura o te lactose hei kūhuka me te galactose. Mā te waikura o te PNPG e te beta-galactosidase ka tukuna he ngota p-nitrophenol (pNP), ka taea te kimi mā te tirotiro ine-ira nā tōna tae kōwhai.

Te tirotiro i ngā aukati me ngā kaiwhakaoho whākōkī: Ka taea te whakamahi i te PNPG i roto i te tirotiro tere-rahi hei tautuhi i ngā matū e whakarerekē ana i te mahi beta-galactosidase. Mā te ine i te tere o te waikura PNPG i te aroaro o ngā matū whakamātautau rerekē, ka taea e ngā kairangahau te tautuhi i ngā aukati e whakaiti ana i te mahi whākōkī, i ngā kaiwhakaoho rānei e whakarei ake ana i te mahi whākōkī.

Te ako i te tere o te whākōkī: Ko te wairākau o te PNPG mā te beta-galactosidase e whai ana i te tere o Michaelis-Menten, e āhei ai ngā kairangahau ki te whakatau i ngā tawhā whākōkī nui pērā i te tere tauhohenga mōrahi (Vmax) me te pūmau Michaelis (Km). Mā ēnei mōhiohio ka āwhina i te mārama ki te hononga o te whākōkī ki te matū me te whai huatanga o te whakakorikori.

Ngā tono koiora ngota: Ko te Beta-galactosidase, e tapatapahi ana i te PNPG, e whakamahia whānuitia ana hei ira pūrongo i roto i te koiora ngota. He maha ngā wā ka whakamahia te papa PNPG hei kimi me te whakaatu i te whakaaturanga o te ira pūrongo, e whakarato ana i tētahi huarahi māmā me te tairongo ki te aromatawai i te whakaaturanga ira i roto i ngā pūnaha whakamātautau.