p-Nitrophenyl-aL-Fucopyranoside CAS:10231-84-2

Ngā whakamātautau ngohe whākōkī: Ko te pNP-α-L-Fucopyranoside he mea whakamahi noa hei matū hanga hei ine i te ngohe o ngā whākōkī α-L-fucosidase. Ka tapahia e te whākōkī te here glycosidic i waenga i te huka fucose me te rōpū p-nitrophenyl, ka hua ake te tukunga o te p-nitrophenol. Ka taea te ine i te nui o te p-nitrophenol i hangaia mā te whakamahi i te tae me te ine ine, mā te ine ine rānei, kia taea ai te whakatau i te ngohe whākōkī.

Ngā rangahau mō te kaha o te whākōkī: Ka taea te whakamahi i te wairākau o te pNP-α-L-Fucopyranoside hei whakatau i ngā tawhā kaha, pērā i te pūmau Michaelis-Menten (Km) me te tere mōrahi (Vmax) o te mahi α-L-fucosidase. Ka whakaratohia e ēnei tawhā he mōhiohio whai hua mō te whai huatanga me te hononga o te whākōkī ki te papa.

Te tirotiro i ngā aukati: Ka taea te whakamahi i te pNP-α-L-Fucopyranoside hei tirotiro me te aromatawai i ngā aukati pea o te α-L-fucosidase. Mā te ine i te mahi a te whākōkī i te aroaro me te ngaro o ngā matū rerekē, ka taea e ngā kairangahau te tautuhi me te whakaahua i ngā aukati ka taea te whakarerekē i te mahi a te whākōkī.