He tini ngā toa kāore i te mōhiotia i te ao, ā, he āhua noa iho rātou, engari he nui ā rātou mahi ki a tātou. Ko te Proteinase K te "toa kāore i te mōhiotia" i roto i te umanga tātaritanga ngota, ahakoa ki te whakatauritea ki te "nui me te kaha" o te umanga, he tino iti te proteinase K, ā, kua roa tātou e wareware ana ki tōna hiranga. I te pakarutanga mai o te mate urutā hou, kua piki ake te hiahia mō te proteinase K, ā, kua tawhiti te tuku i te kāinga me tāwāhi i te kai, ā, ka kite ohorere te katoa he tino hiranga te proteinase K.
He aha te whakamahinga o te proteinase K?
Ko te Proteinase K he protease serine me te mahi whākōkī proteolytic, ā, ka taea te pupuri i te mahi i roto i ngā momo taiao whānui (pH (4-12.5), te tote-nui te parepare, te pāmahana teitei o te 70°C, me ētahi atu). Hei tāpiri, kāore te mahi a te proteinase K e aukatia e te SDS, te urea, te EDTA, te guanidine hydrochloride, te guanidine isothiocyanate, me ētahi atu, ā, ka taea hoki e te nui o te hopi horoi te whakarei ake i te mahi a te proteinase K. I roto i te maimoatanga hauora (te patu huakita me te moroiti), te kai (te whakangawari i te mīti), te hiako (te whakangawari i te makawe), te hanga waina (te whakamārama waipiro), te whakarite waikawa amino (ngā huruhuru kua pakaru), te tango waikawa karihi, te whakarōpūtanga in situ, me ētahi atu, he maha ngā whakamahinga o te proteinase K. Ko te whakamahinga e whakamahia whānuitia ana ko te tango waikawa karihi.
Ka taea e te Proteinase K te whakawhanake i ngā momo pūmua katoa i roto i te tauira, tae atu ki ngā histones e piri pūmau ana ki ngā waikawa karihi, kia taea ai te tuku i ngā waikawa karihi mai i te tauira ka tukuna ki roto i te tangohanga, hei āwhina i te taahiraa e whai ake nei o te tango me te purenga. I te kitenga o te waikawa karihi huaketo, ko te proteinase K tētahi o ngā wāhanga nui o te otinga tauira huaketo. Ka taea e te Proteinase K te pakaru me te whakakore i te pūmua hipoki o te huaketo, he mea haumaru ake i te wā kawe me te kitenga; hei tāpiri, ka taea hoki e te proteinase K te whakaheke i te RNase, ka ārai i te whakahekenga o te RNA huaketo, ā, ka āwhina i te kitenga o te waikawa karihi.
Te rongonui o te proteinase K i te pō kotahi
Ahakoa i roto i te ao rangahau pūtaiao, i roto rānei i te ao IVD, ko te tango waikawa karihi te whakamātautau tino taketake, nō reira he mea tino nui tonu te proteinase K. Heoi, i ngā wā o mua, kāore i te tino mōhiotia te proteinase K i tōna tūranga. Ko te nuinga o tēnei nā te mea he tino pumau te whanaungatanga tuku me te tono o te proteinase K. He iti noa te hunga e whakaaro ana he raruraru te tuku o te proteinase K.
I te pakarutanga mai o te mate urutā hou, kua piki ake te hiahia mō te whakamātautau waikawa karihi. I te mutunga o Hune 2020, kua oti i Haina tata ki te 90 miriona whakamātautau karauna hou, ā, he nui ake te mataku o tēnei tau i te ao whānui. I roto i ngā whakamātautau tango waikawa karihi, ko te kukū mahi o te proteinase K he tata ki te 50-200 μg/mL. I te nuinga o te wā, e 100 μg o te proteinase K te tango i tētahi tauira o te waikawa karihi. I te whakamahinga tūturu, hei whakanui ake i te whai huatanga o te tango waikawa karihi, he maha ngā wā ka whakamahia te Proteinase K i roto i te nui ake. Nā te kitenga waikawa karihi o te mate korona hou kua nui ake te hiahia mō te proteinase K. I tere te pakaru o te toenga tuku me te tono taketake o te proteinase K, ā, i roto i te pō kotahi ka noho te proteinase K hei rauemi ārai mate urutā nui.
Ngā uauatanga o te whakaputanga o te proteinase K
Ahakoa kua whakanuia te uara nui o te proteinase K e te tangata i te whanaketanga o te mate urutā, he mea whakama te mea nā te iti rawa o te mahi a te proteinase K, he iti noa ngā kamupene o te rohe kua uru ki te hanga proteinase K. I te hiahia o te tangata ki te whakatū i te hanga proteinase K. I te wā o te tukanga hanga, i kitea he tino motuhake te proteinase K. He tino uaua ki te whakawhānui i te kaha hanga o te proteinase K i roto i te wā poto.
Ko te whakaputanga nui o te proteinase K e pāngia ana e ngā uauatanga e whai ake nei:
1. Te iti o te whakapuakitanga
Ka taea e te Proteinase K te whakangaro i te nuinga o ngā pūmua, ā, ka pāngia te pūtau manaaki e te paitini. Nō reira, he tino iti te taumata o te proteinase K. Ko te tirotiro i ngā pūnaha me ngā momo e tino whakaputa ana i te proteinase K me roa ake te huringa.
2. Ngā toenga o ngā tae me ngā waikawa karihi
Ka whakaurua mai he nui o te tae me ngā toenga waikawa karihi manaaki i te whakapūkara nui. He uaua ki te tango i ēnei poke mā te tukanga purenga māmā noa iho, ā, ka piki ake te utu me te tere whakaora i te purenga uaua.
3. Te kore pumau
Kāore i te tino pumau te Proteinase K, ka taea e ia te whakawhanake i a ia anō, ā, he uaua ki te penapena pumau i te 37°C mō te wā roa me te kore he kaihoko tiaki.
4. Ngāwari ki te whakapūpū
I te wā e whakarite ana i te paura maroke-tio o te proteinase K, kia nui ai te ihirangi totoka o te proteinase K i roto i te paura maroke-tio, me tāpiri he kaihoko tiaki maroke-tio i te kukū teitei, engari ina tae te kukū o te proteinase K ki te 20mg/mL me runga ake, ka ngāwari te whakakotahitanga ki te hanga i tētahi putunga, ka nui ai ngā uauatanga ki te maroke-tio o te proteinase K he nui te ihirangi totoka.
5. Te haumitanga nui
He kaha te mahi a te Proteinase K ki te protease, ā, ka taea e ia te whakawhaiti i ētahi atu protease i roto i te taiwhanga. Nō reira, me whai wāhi whakaputa motuhake, taputapu, me ngā kaimahi mō te rangahau, te whanaketanga me te whakaputanga o te proteinase K.
Te otinga proteinase K a XD BIOCHEM
He tūāpapa whakaputa me te purenga pūmua pakari tā XD BIOCHEM, ā, he nui ngā wheako ki te whakaputa me te purenga o ngā pūmua recombinant me te arotau i ngā tukanga whakaputa. Nā te tere o te hanganga o tētahi rōpū rangahau me te whanaketanga, kua wikitoria te tukanga whakaputa nui o te proteinase K. Neke atu i te 30 KG te putanga marama o te paura maroke-tio. He pumau te mahi a te hua, he nui te mahi motuhake ki te whākōkī, ā, kāore he toenga cytochrome me te waikawa karihi. Nau mai ki te whakapā atu ki a XD BIOCHEM Tikina he mōkihi whakamātautau (Īmēra:sales@xdbiochem.comWaea: +86 513 81163739).
Kei roto i ngā otinga hangarau a XD BIOCHEM
Mā te whakamahi i te whakaurunga plasmid maha-tārua, ka tīpakohia ngā momo whakaputanga teitei me te taumata whakaputanga o te 8g/L, ka whakakorea te raruraru o te taumata whakaputanga iti o te proteinase K.
Mā te whakatūnga o tētahi tukanga purenga maha-taahiraa, i angitu te tango i ngā toenga cytochrome me ngā toenga waikawa karihi o te proteinase K i raro i te uara paerewa.
Mā te tātaritanga tere-whakaputa o ngā whakatakotoranga arai tiaki, i tīpakohia he arai ka taea te pupuri pūmau i te proteinase K i te 37°C.
Mā ngā arai tirotiro ka whakakorea te raruraru he ngāwari te kohikohi me te taupatupatu o te proteinase K i ngā kukū tiketike, ā, ka whakatakoto i te tūāpapa mō te whakamaroke-tio o te proteinase K me te nui o te ihirangi totoka.
Tauira XD BIOCHEM proteinase K
Whakamātautau pumau XD BIOCHEM proteinase K: kāore he huringa nui o te ngohe i muri i te 80 rā i te pāmahana rūma
Whakamātautau pumau XD BIOCHEM proteinase K: kāore he huringa nui o te ngohe i muri i te 80 rā i te pāmahana rūma.
Te whakataurite i te pānga tango waikawa karihi o te XD BIOCHEM proteinase K me ngā hua whakataetae. I roto i te tukanga tango waikawa karihi, ka whakamahia te XD BIOCHEM me te proteinase whakataetae K. He teitei ake te whai huatanga tango o te XD BIOCHEM proteinase K, ā, he iti iho te uara Ct o te ira ūnga.
Wā tuku: Hakihea-31-2021