Ko te Dithiothreitol (DTT), CAS: 3483-12-3, he matū rangahau pūtaiao e whakamahia whānuitia ana, e whakamahia ana hei matū whakaiti mō te sulfhydryl DNA, hei matū tango tiaki, me te whakaiti i ngā here disulfide i roto i ngā pūmua. He mea nui te mahi a tētahi momo tāpiri matomato hou ki te whakapai ake i te mahi a te pākahiko.
He kaha te whakaiti i te Dithiothreitol (DTT), ā, ko tōna kaha whakaiti i te nuinga o te wā nā te pumau o te āhua o te mowhiti ono-mema (kei roto ngā here disulfide) i roto i tōna āhua waikura. Ko te whakaiti i te here disulfide noa e te dithiothreitol he rua ngā tauhohenga whakawhiti here sulfhydryl-disulfide. Ka pāngia te mana whakaiti o te dithiothreitol (DTT) e te uara pH, ā, ka taea anake te whai pānga whakaiti ina nui ake te uara pH i te 7. Nā te mea ko ngā anion thiolate kua tangohia te proton anake ka tauhohe, engari kāore ngā mercaptan, ā, ko te pKa o ngā rōpū mercapto he 8.3 te nuinga.
Ko te Dithiothreitol (DTT) e whakamahia whānuitia ana hei whakaiti i ngā here disulfide o ngā ngota pūmua me ngā polypeptides. E whakamahia ana hei kaihoko tiaki pūmua sulfhydryl, ā, e whakamahia ana hoki i roto i ngā whakaritenga kano hei ārai i ngā toenga pūmua cysteine mai i te hanga disulfides intramolecular me intermolecular. He mea nui tēnei. I roto i te tukanga kimi waikawa karihi, ka taea e te dithiothreitol (DTT) te whakangaro i ngā here disulfide i roto i te pūmua RNase, te whakakore i te āhua o te RNase, me te whakahaere i ngā whakamātautau pēnei i te hanga whare pukapuka RNA me te whakanui i te RNA. E whakamahia ana hoki te Dithiothreitol (DTT) hei rongoā ārai mate hei tiaki i ngā pūtau me ngā kiko, hei ārai i te irahiko, me ētahi atu.
Heoi, kāore e taea e te dithiothreitol (DTT) te whakaiti i ngā here disulfide kua mau ki roto i te hanganga pūmua (kāore e taea te uru atu ki te whakarewa). Ko te whakaiti i aua here disulfide me whakakore i te pūmua i te tuatahi.
Hei aukati i te pānga whakawhiti o ngā pākahiko lithium-whanariki me te whakapai ake i te mahi hikohiko o ngā pākahiko lithium-whanariki, ngana ki te whakamahi i te dithiothreitol (DTT) hei kaihoko kutikuti hei kutikuti i ngā polysulfides ota teitei hei ārai i a rātou kei memeha. Ka konatunatua te Threitol (DTT) ki roto i te pepa nanotube waro maha-pakipa (MWCNTs) hei whakarite i tētahi paparanga DTT. Ka whakanohoia te paparanga DTT ki waenganui i te pepa hiko pai me te wehewehe o te haurua-pūtau pātene lithium-whanariki, me te mātotoru mata kawe-whanariki o te pepa hiko pai Tata ki te 2mg/cm2. Ka whakaū ngā hua tirotiro SEM kei te horapa ōrite te DTT ki runga i te mata me ngā āputa o te pepa MWCNTs. E whakaatu ana ngā hua whakamātautau hikohiko ko te pākahiko lithium-whanariki me te hanganga hanawiti DTT he kaha tuku tuatahi motuhake o te 1288 mAh/g i te tere o te 0.05C. Mō te wā tuatahi, kua tata te pai o te coulombic ki te 100%, ā, ko te kaha motuhake i te wā e utu ana, e tuku ana i ngā tere 0.5C, 2C, me te 4C ka eke ki te 650mAh/g, 600mAh/g, me te 410mAh/g. Mā te whakaurunga o te hanganga hanawiti DTT ka taea te kutikuti i ngā polysulfides ota-teitei. Ka ārai i te hekenga ki te irahiko lithium kino, ka aukati i te pānga whakawhiti me te whakapai ake i te pumau huringa me te pai o te coulomb o ngā pākahiko lithium-whanariki.
He mea tika kia mōhiotia ko te dithiothreitol (DTT) he matū paitini. Hei tauira, i te aroaro o ngā konganuku whakawhiti, ka taea e te dithiothreitol (DTT) te whakararu i ngā ngota koiora. I taua wā anō, ka taea hoki e te dithiothreitol (DTT) te whakanui ake i te paitini o ētahi matū kei roto ko te arsenic me te mercury. He kakara kino te Dithiothreitol (DTT), ka kino pea ki te hauora nā te manawa me te pa ki te kiri. Nō reira, me tiaki i te wā e whakamahia ana, me mau kopare kanohi, karapu me ngā mōhiti kanohi, me te mahi i roto i te taupoki auahi.
Ko te Thithreitol (DTT) hei kaihopu kutikuti i roto i ngā pākahiko lithium-whanariki
E kiia ana te pākahiko lithium-whanariki he pūnaha pākahiko he nui te kaha nā te mea he nui te kaha o te pūngao me te tiaki taiao. Heoi, ko te "pānga whakawhiti" o ngā polysulfides ka arahi ki te roa o te huringa me te tukunga-ake-ake nui, ka whakawhāiti i tana whakamahinga.
Ka taea te tāpiri i te Thiothreitol (DTT) ki te pākahiko hei kaihopu kutikuti. Ka taea e ia te kutikuti tere i ngā here disulfide i te pāmahana rūma, te kutikuti i ngā polysulfides ota-teitei hei ārai i tō rātou memeha, te aukati i te pānga whakawhiti, me te whakanui ake i te mahi hikohiko o ngā pākahiko whanariki lithium.
Dithiothreitol (DTT) hei tāpiringa irahiko i roto i ngā pākahiko konumohe/hau kawakore
I roto i ngā pākahiko konumohe/hau kawakore, ka taea e te dithiothreitol te hanga i tētahi paparanga tiaki ōrite, pumau hoki mā roto i ngā hononga honohono hihiri i runga i te mata o te anode konumohe, ka aukati i te waikura-ā-ia anō o te anode konumohe, ā, ka whakapai ake i tana mahi.
Wā tuku: Hakihea-31-2021