HEPES CAS:7365-45-9 Utu Kaihanga

Ko te HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) he pūhui koiora e whakamahia whānuitia ana i roto i ngā rangahau koiora me ngā rangahau matū. E mōhiotia ana mō tōna kaha ki te pupuri i te pH pumau i roto i ngā otinga wai, e pai ai mō ngā tono maha e pā ana ki ngā tukanga pūtau me ngā tukanga whākōkī. He matū zwitterionic te HEPES, arā, he utu pai me te utu kino kei runga i ngā rōpū mahi motuhake, e āhei ai ki te ātete i ngā huringa o te pH i puta mai i te tāpiri o ngā waikawa, o ngā turanga rānei. He maha te whakamahinga i roto i te ahurea pūtau, ngā whakamātautau whākōkī, ngā rangahau pūmua, ngā whakamātautau electrophoresis, me ngā hanganga rongoā.

Tonohia, Tonoa rānei he Tāpaetanga

Taipitopito Hua

Ngā Tohu Hua

Te Whakamahinga me te Pānga

Te ārai i te pH: E whakamahia whānuitia ana te HEPES hei kaihoko ārai hei pupuri i te pH pumau i roto i ngā pāpāho whakatipu pūtau me ngā whakamātautau koiora. He whai hua tana mahi i roto i te awhe pH ā-tinana o te 6.8 ki te 8.2, he mea pai mō te pupuri i ngā āhuatanga tino pai mō ngā tukanga koiora.

Ahurea pūtau: He mea noa te tāpiri i te HEPES ki ngā pāpāho arumoni pūtau hei whakau i te taumata pH, hei whakarato i tētahi taiao e tika ana mō te tipu o te pūtau me te pupuri i te oranga o te pūtau. Ka āwhina i te ārai i ngā rerekētanga pH ka pā kino ki te whanonga o te pūtau me ngā hua o ngā whakamātautau.

Ngā whakamātautau whākōkī: He maha ngā wā ka whakamahia te HEPES hei ārai i ngā tauhohenga whākōkī nā tōna kaha ki te pupuri i tētahi pH motuhake. Ka āwhina i te pupuri i te mahi whākōkī me te pumau tino pai, ā, ka whakarei ake i te tika me te pono o ngā whakamātautau whākōkī.

Ngā rangahau pūmua: E whakamahia ana te HEPES i roto i ngā whakamātautau e pā ana ki te pūmua, tae atu ki te purenga pūmua, te whakatiotanga pūmua, me te tātari hanganga pūmua. Ka āwhina ki te whakau i te pH me te pupuri i te takai me te pumau o te pūmua i roto i ēnei whakamātautau.

Hikohikohiko: Ka kitea te whakamahinga o te HEPES i roto i ngā tikanga hikohikohiko ira, pērā i te SDS-PAGE me te hikohikohiko ira agarose. Ka whakamahia hei kaihoko ārai i roto i te ārai e rere ana, e whakarato ana i tētahi taiao pH pumau mō te wehenga me te tātari i ngā ngota koiora.

Ngā whakatakotoranga rongoā: Ka whakamahia te HEPES i roto i te whakatakotoranga o ngā hua rongoā me ngā hua hangarau koiora. Ka āwhina ki te pupuri i te pumau me te pono o ngā kai hohe, kia mau ai te whai huatanga me te roa o te wā e rongoa ai.

Te Tākai Hua:

Ngā Mōhiotanga Tāpiri:

Te hanganga	C8H18N2O4S
Whakamātautau	99%
Āhua	Paura mā
Nama CAS	7365-45-9
Te tākai	Iti, nui hoki
Te Ora o te Wāhi	2 tau
Rokiroki	Penapena ki te wāhi hauhautanga, maroke hoki
Tiwhikete	ISO.