Gly-Pro p-nitroanilide p-toluenesulfonate tote CAS:65096-46-0

Pānga: Ko te tote p-nitroanilide Gly-Pro p-nitroanilide p-toluenesulfonate he mea hanga motuhake hei papa mō ngā peptidase motuhake ki te proline. Ka tapahia e ēnei whākōkī te here peptide i waenga i ngā toenga glycine (Gly) me te proline (Pro), ka tukuna te p-nitroanilide (pNA) hei hua. Ka mimiti te ngota pNA i te mārama i tētahi roangaru motuhake, ka taea ai te ine i te pNA kua tukuna me te aromatawai i te mahi whākōkī.

Te Whakamahinga: E whakamahia ana te tote Gly-Pro p-nitroanilide p-toluenesulfonate i roto i ngā momo rangahau, tae atu ki te matū koiora, te whākōkī, me te kimi rongoā, mō ngā whakamahinga e whai ake nei:

Ngā whakamātautau ngohe whākōkī: E whakamahia whānuitia ana hei ine i te ngohe o ngā peptidase motuhake ki te proline, tae atu ki ngā dipeptidyl peptidase (DPP) me ngā prolyl endopeptidase (PEP).

Ngā aukati whākōkī tirotiro: Ka taea te whakamahi i te tote Gly-Pro p-nitroanilide p-toluenesulfonate hei aromatawai i te pūmanawa aukati o ngā matū, o ngā rongoā rānei ki te mahi peptidase motuhake ki te proline. Ko te hekenga o te tukunga o te pNA e tohu ana i te aroaro o tētahi aukati.

Ngā rangahau mō te kaha o te whākōkī: Mā te whakarerekē i te kukū o te tote Gly-Pro p-nitroanilide p-toluenesulfonate me te ine i te tere o te tukunga pNA, ka taea te whakatau i ngā tawhā kaha o te whākōkī pēnei i te Km (pūmau Michaelis-Menten) me te Vmax (tere mōrahi).

Ngā tono tātaritanga: Ka whai pānga pea te mahi a ngā peptidase motuhake ki te proline i roto i ngā whakamātautau tātaritanga mō ngā mate me ngā āhuatanga maha, pērā i ngā mate urutā me ngā mate pukupuku. Ka taea te whakamahi i te tote Gly-Pro p-nitroanilide p-toluenesulfonate hei taputapu hei aromatawai i ngā taumata mahi a ēnei whākōkī i roto i ngā tauira tūroro.