Te konupūmā ira-whakarewa (te konupūmā G418) CAS:108321-42-2

I roto i ngā taiwhanga, e whakamahia whānuitia ana te geneticin disulfate (G418 sulfate) i roto i ngā whakamātautau ahurea pūtau hei tīpako me te pupuri i ngā raina pūtau kei roto ngā kawe whakaputanga me ngā ira ātete, pērā i te neomycin phosphotransferase. Ka taea e ēnei pūtau te ora i te aroaro o te geneticin disulfate nā te whakaputanga o te ira ātete, e āhei ai ngā kairangahau ki te wehe me te ako i ngā taupori pūtau kua whakarerekētia te ira. Ka whakamahia e ngā kairangahau te geneticin disulfate i roto i ngā tono maha, tae atu ki te whakaputanga raina pūtau pumau, ngā tikanga whakatika ira pērā i te CRISPR-Cas9, me te whakaputa pūmua recombinant. Mā te whakauru i te geneticin disulfate ki roto i te reo ahurea, ka taea te whakanui ake, te wehe rānei i ngā taupori pūtau motuhake i runga i tō rātou kaha ki te ora i te kōwhiringa paturopi. He mea nui te arotau i te kukū me te roa o te maimoatanga geneticin disulfate hei whakatutuki i te pēhanga whiriwhiri me te whakaiti i te cytotoxicity me ngā pānga kore-whāinga ki ngā pūtau kāore i whakawhitia. Mā te aroturuki tupato i te oranga o te pūtau, ngā āhuatanga tipu, me ngā tauira whakaputanga ira ka āwhina i te whakarite i ngā putanga whakamātautau angitu i te wā e whakamahi ana i te geneticin disulfate i roto i ngā rangahau ahurea pūtau. Me whai ngā kairangahau i ngā tikanga kua whakatūria mō te whakamahinga o te geneticin disulfate, tae atu ki te horopeta tika, te whakahaere, me ngā tikanga whakakorenga, hei pupuri i ngā paerewa haumaru taiwhanga me te whakatairanga i ngā hua pūtaiao tika. Ko te mahi tahi a ngā kaipūtaiao, ngā kaimahi taiwhanga, me ngā mana whakahaere e tautoko ana i te whakamahinga whai kawenga me te whai hua o te geneticin disulfate i roto i te rangahau koiora ngota, e whakatairanga ana i tō tātou māramatanga ki ngā tukanga pūtau me ngā tikanga whakahaere ira.