FLUORESCEIN MONO-BETA-D-GALACTOPYRANOSIDE CAS:102286-67-9

Ko te Fluorescein mono-beta-D-galactopyranoside (FMG) he ngota e whakamahia whānuitia ana i roto i ngā rangahau koiora hei tūāpapa hei kimi i te aroaro me te mahi a te whākōkī beta-galactosidase. Ko te FMG he pūtake o te lactose huka, ā, ka honoa ki te ngota fluorescein.

Ko te pānga matua o te FMG ko te whakawhaiti motuhaketia e te beta-galactosidase, he whākōkī e wawahi ana i te lactose ki te galactose me te glucose. Mā tēnei whakawhaiti whākōkī o te FMG ka tukuna te fluorescein, ka tukuna he tohu fluorescence kaha.

Ko te whakamahinga matua o te FMG he kimi me te ine i te mahi a te beta-galactosidase i roto i ngā tauira maha. Kei roto i te maha o ngā rauropi tēnei whākōkī, tae atu ki ngā huakita me ngā pūtau mammal, ā, ka taea e tōna mahi te tohu i ngā tukanga pūtau me ngā ara pākia.

Mā te whakamahi i te FMG hei tūāpapa, ka taea te ine i te mahi beta-galactosidase mā te aroturuki i te fluorescein kua tukuna. Ka taea te mahi i tēnei ine i roto i ngā momo whakamātautau, tae atu ki ngā whakamātautau in vitro me ngā rangahau whakaahua pūtau ora.

Hei tāpiritanga, ka taea te whakamahi i te FMG hei taputapu hei ako i te tohatoha me te tauwāhi o te beta-galactosidase i roto i ngā pūtau. Mā te whakamahi i ngā tikanga karuārai hihi, ka taea e ngā kairangahau te tiro i te hihi e tukuna ana e te FMG i te wā o te waikura, kia taea ai e rātou te whai i te mahi ā-wāhi me te wā o te beta-galactosidase.