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Ninhydrin hydrate CAS:485-47-2 Utu Kaihanga
He matū te ninhydrin hydrate e whakamahia whānuitia ana i roto i te tātaritanga taihara me te matū waro. He paura tioata kore tae, he kōwhai mārama rānei, ka wairewa ki te wai me ngā whakarewa waro.
E mōhiotia ana te Ninhydrin hydrate mō tōna kaha ki te tauhohe ki ngā waikawa amino me ngā amine tuatahi, ka puta he tae papura-kikorangi e kitea ana. He mea whakamahi noa tēnei tauhohenga i roto i te tātari tapumati ture hei tiro me te whakarei ake i te kitea o ngā tapumati huna i runga i ngā mata pūwero. I te nuinga o te wā ka rehuhia te otinga ninhydrin ki runga i te mata, ā, ka whanake haere ngā tapumati i te wā e tauhohe ana te ninhydrin ki ngā waikawa amino kei roto i ngā toenga tapumati.
I roto i te matū waro, ka whakamahia te ninhydrin hydrate hei matū hei kimi me te tātari i ngā waikawa amino, ngā peptide, me ngā pūmua. Ka taea te whakamahi hei whakatau i te aroaro me te ine i te nui o ngā waikawa amino i roto i te tauira. Ko te tauhohenga ki te ninhydrin ka hua ake he hua tae, ka taea te ine mā te spectrophotometric hei whakatau tata i te kukū o te analyte.
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D-Luciferin CAS:2591-17-5 Utu Kaihanga
Ko te D-Luciferin he matū iti e tuku mārama ana, e kitea nuitia ana i roto i ngā rauropi koiora pērā i ngā pūngāwerewere, ngā huakita, me ngā rauropi moana. Koinei te wāhanga matua o te tauhohenga luciferase, he tukanga matū koiora e whakaputa ana i te mārama.
He maha ngā wā ka whakamahia te D-Luciferin hei tūāpapa i roto i ngā whakamātautau bioluminescence me ngā tikanga whakaahua, ka puta te mārama ka taea te kite me te ine i tana tauhohenga ki te luciferase. Nā tēnei ka waiho hei taputapu whai hua i roto i ngā momo rangahau, tae atu ki te koiora ngota, te kimi rongoā, me te whakaahua koiora.
Nā ōna āhuatanga whatu ahurei, kua whakamahia whānuitia te D-Luciferin i roto i ngā rangahau e pā ana ki te whakaaturanga ira, ngā taunekeneke pūmua-pūmua, me te tohu pūtau. He tikanga tairongo, kāore e pokanoa ki te tirotiro i ngā tukanga koiora i te wā tonu.
Hei tāpiritanga, kua whakawhanakehia ngā momo whakawhiti me ngā momo whakawhiti D-Luciferin, e āhei ai ngā whakarerekētanga me ngā whakamahinga rerekē. Mā ēnei momo whakawhiti kua whakarerekētia ka taea te whakaputa i ngā roangaru rerekē o te māramatanga, te whakapai ake i te pumau, me te whakapai ake i te urunga ki roto i te kiko.
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waikawa p-hydroxybenzoic, monosodium CAS: 114-63-6
Ko te waikawa p-Hydroxybenzoic, monosodium (e mōhiotia ana ko te konutai 4-hydroxybenzoate) he matū kei roto ko te tātai ngota C7H5NaO3. He tote konutai i ahu mai i te waikawa p-hydroxybenzoic, he matū waro tūturu e kitea ana i roto i ngā tipu pēnei i ngā hua me ngā huawhenua.
Ko te waikawa p-Hydroxybenzoic, monosodium, e whakamahia ana i roto i ngā ahumahi maha nā ōna āhuatanga patu huakita. He mea whakamahi noa hei tiaki i roto i ngā kai, inu, whakapaipai, me ngā hua tiaki whaiaro hei aukati i te tipu o ngā huakita, harore, me ngā rewena. Ka āwhina i te whakaroa i te oranga o ēnei hua mā te ārai i te pirau me te tiaki i tōna kounga.
I roto i ngā mahi rongoā, ka whakamahia te waikawa p-Hydroxybenzoic, te monosodium, hei tāpiri i roto i ngā rongoā ā-waha me ngā whakatakotoranga kiri. Ka āwhina ōna āhuatanga patu huakita ki te pupuri i te pumau me te koretake o ēnei hua, kia mau ai te haumaru me te whai huatanga.
Hei tāpiritanga, he āhuatanga ārai-mate kei roto i te waikawa p-Hydroxybenzoic, arā, te monosodium, e whai hua ai ki te ārai i te kino o te waikura i roto i ngā momo hua. Ka taea e ia te āwhina ki te tiaki i te pirau o ngā kai me te whakaroa i te roa o te ora o te hua..
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4-Nitrophenyl phosphate disodium tote hexahydrate CAS: 4264-83-9
Ko te 4-Nitrophenyl phosphate disodium salt hexahydrate he matū e whakamahia whānuitia ana hei tūāpapa mō te kimi i te mahi a ngā whākōkī phosphatase. He rite ki te paura mā ki te paura mā, ā, he tino wairewa ki te wai. Ina pāngia e ngā whākōkī phosphatase, ka pāngia e te tauhohenga ka puta he tae kōwhai, ka taea te ine mā te spectrophotometric. Ka kitea ngā tono o tēnei matū i roto i ngā whakamātautau matū koiora me ngā kete tātaritanga mō te kimi me te ine i te mahi phosphatase i roto i ngā tauira..
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Methylphenazinium methosulfate CAS:299-11-6
Ko te Methylphenazinium methosulfate (MPMS) he matū hohe-whakaora e whakamahia whānuitia ana hei kaikawe irahiko i roto i ngā rangahau matū koiora me te koiora ā-tinana. He tote kei roto ko te methylphenazinium cation (he matū heterocyclic) me te anion methosulfate.
He maha ngā wā ka whakamahia te MPMS hei whakakapi i ngā kaikawe irahiko tuku iho, pērā i te ferricyanide, te phenazine ethosulfate rānei, nā tōna pumau me te wairewatanga teitei i roto i te wai. He pai ngā āhuatanga redox, e āhei ai ki te tango me te whakawhiti i ngā irahiko i roto i ngā tauhohenga whākōkī.
Ko tētahi o ngā whakamahinga matua o te MPMS ko ngā whakamātautau e uru ana ki te ine i te whakawhiti irahiko, te mahi whākōkī rānei. He maha ngā wā ka whakamahia ngātahitia me tētahi pūnaha whākōkī hei aroturuki i te whakawhiti irahiko i waenga i ngā wāhanga rerekē. Ka taea te kite i te whakahekenga o te MPMS mā te ine irahiko, ina rerekē tōna mimiti hei hua o ngā tukanga whakawhiti irahiko.
Ka whakamahia hoki te MPMS i roto i ngā rangahau e pā ana ki te manawa mitochondrial me te phosphorylation oxidative. Ka taea e ia te mahi hei kaiwhiwhi irahiko horihori, e āhei ai ngā kairangahau ki te rangahau i te mahi me te whakahaere i ēnei tukanga i roto i ngā pūnaha koiora rerekē.
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4-Nitrophenyl-alpha-L-fucopyranoside CAS:10231-84-2
Ko te 4-Nitrophenyl-alpha-L-fucopyranoside he matū matū nō te whānau glycosides. He ngota huka fucose tōna e piri ana ki tētahi rōpū 4-nitrophenyl. He mea whakamahi noa tēnei matū hei matū i roto i ngā whakamātautau whākōkī hei ako i te mahi a ngā fucosidases, arā, ngā whākōkī e uru ana ki te wawahi i ngā ngota kei roto te fucose. Ina pāngia e te whākōkī fucosidase, ka pakaru te 4-nitrophenyl-alpha-L-fucopyranoside, ka puta te tukunga o te 4-nitrophenol, ka taea te ine i te rahinga mā te whakamahi i te spectrophotometry. He tino whai hua tēnei matū i roto i ngā rangahau e pā ana ki te mahi whākōkī, te motuhaketanga o te matū, te tirotiro aukati, me te kinetics o ngā whākōkī fucosidase.
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N-Ethylmaleimide CAS:128-53-0 Utu Kaihanga
Ko te N-Ethylmaleimide (NEM) he matū rauropi iti e whakamahia whānuitia ana i roto i te matū koiora me te rangahau koiora ngota. He aukati motuhake tēnei i ngā rōpū pūmua sulfhydryl (thiol) mā te whakarerekē me te aukati i ā rātou mahi. He tino tauhohe te NEM ki ngā rōpū sulfhydryl, pērā i ngā mea e kitea ana i roto i te waikawa amino cysteine, ā, ka taea te tauhohe ki ngā rōpū sulfhydryl kore utu me ngā mea kei roto i ngā pūmua. Nā tēnei ka waiho te NEM hei taputapu whai hua mō te ako i te mahi pūmua, ngā taunekeneke pūmua-pūmua, me te mahi whākōkī. Kua whakamahia ōna āhuatanga aukati i roto i te whānuitanga o ngā tono, tae atu ki te proteomics, te enzymology, te koiora hanganga, me te kitenga rongoā.
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5-Waikawa Sulfosalicylic dihydrate CAS:5965-83-3
Ko te 5-Sulfosalicylic acid dihydrate he matū matū e whakamahia whānuitia ana i roto i ngā taiwhanga matū koiora me ngā taiwhanga rongoā. He matū tioata mā e tino wairewa ana i roto i te wai. Ko te 5-Sulfosalicylic acid dihydrate he matū i ahu mai i te waikawa salicylic, ā, ko tōna tauira ngota ko C7H6O6S. He maha ngā wā ka whakamahia hei matū mō te whakapūtanga pūmua, ā, ka taea te whakamahi hei ine i ngā pūmua i roto i ngā tauira koiora, pērā i te mimi me te toto. Hei tāpiri, ka taea te whakamahi hei whakatau i ngā momo matū, tae atu ki ngā waikawa karihi, ngā whākōkī, ngā homoni, me ngā rongoā. Ko te āhua dihydrate o te matū e tohu ana kei roto e rua ngā ngota wai mō ia ngota o te waikawa.
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Pāhare pākawa iodide CAS:7681-11-0
Ko te pāhare pākawa iodide (KI) he matū kore-waro e titoa ana i ngā kationa pāhare pākawa (K+) me ngā anionā pāhare pākawa iodide (I-). He totoka mā, he tioata, he tino wairewa ki te wai. He maha ngā whakamahinga me ngā tono a te pāhare pākawa iodide nā ōna āhuatanga.
Ko tētahi o ngā whakamahinga matua o te pāhare pākawa iodide ko te rongoā. He mea whakamahi noa hei tāpiringa hei rongoā me te ārai i ngā āhuatanga e pā ana ki te korenga o te iodine, pērā i te goiter, te kore taurite o ngā homoni tairoi, me ētahi momo mate pukupuku tairoi. Ka taea hoki te whakamahi hei rongoā ohorere mō te pānga o te irahiko, nā te mea ka āwhina i te aukati i te mimiti o te iodine irahiko e te repe tairoi.
He whakamahinga anō hoki te pāhare pākawa iodide i roto i ngā taiwhanga. E whakamahia ana hei matū tātari hei kimi i te aroaro o ētahi huānga, pērā i te mata me te mercury, mā te hanganga o ngā matū kōwhai kore e rewa. Hei tāpiri, ka taea te whakamahi hei pūtake o ngā katote iodide i roto i ngā tauhohenga matū.
I roto i te ao tunu kai, ka tāpirihia te pāhare pākawa iodide ki te tote tēpu (tote whai iodide) hei ārai i te korenga o te iodide i roto i ngā taupori. He maha ngā tāngata o te ao e kai ana i te tote whai iodide hei puna kai mō te iodide.
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8-Anilino-1-naphthalenesulfonic waikawa tote ammonia CAS:28836-03-5
Ko te tote ammonia 8-Anilino-1-naphthalenesulfonic acid he matū e whakamahia whānuitia ana hei tae kanapa. He hanganga kei roto ko te rōpū aniline e piri ana ki te ngota waikawa naphthalenesulfonic me te tote ammonia. Ka wairewa tēnei matū i roto i te wai, ā, he kaha te mimiti me te tuku i roto i te whānuitanga mārama e kitea ana.
Nā ōna āhuatanga kanapa, ka whakamahia te tote ammonium 8-Anilino-1-naphthalenesulfonic acid hei tirotiro, hei tohu rānei i roto i ngā rangahau matū koiora me te koiora ā-tinana. Ka taea te whakamahi hei kite i ngā huringa o te pH, ngā huringa āhua o ngā pūmua, ngā taunekeneke pūmua-ligand, me te aroaro o ētahi katote.
Ina whakaohokia e te roangaru mārama e tika ana, ka tukuna e tēnei pūhui he kikorangi kanapa, e ngāwari ai te kite me te ine. Nā ōna āhuatanga hihi, he taputapu whai hua tēnei i roto i ngā wāhanga rangahau pēnei i te koiora ngota, te matū koiora, me te koiora pūtau.
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Konutai dodecyl sulfate CAS: 151-21-3
Ko te konutai dodecyl sulfate (SDS) he matū horoi anionic e whakamahia whānuitia ana i roto i ngā ahumahi me ngā mahi rangahau. He totoka mā, he paura rānei e wairewa ana i roto i te wai, ā, ka hanga he pahuka kaha, pumau ina whakaohokia. E mōhiotia ana te SDS mō tōna kaha ki te wairewa me te whakakore i te āhua o ngā pūmua, ā, he mea whai hua i roto i te tango pūmua, te purenga, me ngā tikanga hikohiko. Hei tāpiri, he whānuitia te whakamahinga o te SDS hei hopi horoi i roto i ngā hua tiaki whare me te tiaki whaiaro, tae atu ki ngā mahi horoi ahumahi. He wāhanga matua hoki i roto i te maha o ngā whakamātautau koiora matū me te koiora ngota, e whakamahia ana mō te pakaru o te pūtau, te wehe i te DNA, me te hei kaihoko whakakore i roto i te hikohiko gel.
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BCA-2K CAS:207124-63-8 Utu Kaihanga
Ko te BCA-2K he whakarāpopototanga mō te Beta-Carotene Apocarotenoid-2-Ketolase. He whākōkī tēnei e whai wāhi nui ana ki te hanga i ngā tae tipu e kiia nei ko ngā apocarotenoids. I ahu mai ēnei tae i te ngota beta-carotene matua, ā, e whai wāhi ana ki ngā tukanga koiora maha i roto i ngā tipu.
Mā te BCA-2K ka whakakorikori i te hurihanga o te beta-carotene hei apocarotenoid motuhake mā te tāpiri i tētahi rōpū ketone ki te ngota. He mea nui tēnei tauhohenga whākōkī mō te whakaputanga o ngā apocarotenoid motuhake me ngā mahi koiora rerekē i roto i ngā tipu.
Kua kitea he mahi nui tā ngā apocarotenoid i hangaia e te BCA-2K ki te whanaketanga o ngā tipu, ki ngā urupare ahotea, me te ārai i ngā mate me ngā pepeke. Ka taea hoki e rātou te mahi hei ngota tohu e uru ana ki ngā tukanga ā-tinana.
Ko te rangahau i te BCA-2K me tōna tūranga i roto i te hanga apocarotenoid ehara i te mea he mea nui anake mō te mārama ki te koiora tipu engari he tono pea anō hoki ki ngā wāhanga pēnei i te whakapai ake i ngā hua, te whakatipu tipu, me te whakawhanake i ngā hua taiao he painga rongoā, he painga kai rānei.
