DNase I, mai i te Pancreas Bovine CAS:9003-98-9

Ko te DNase I, i ahu mai i te repe kau, he whākōkī e tapatapahi ana i ngā ngota DNA mā te whakakōkō i ngā here phosphodiester i roto i te tuarā DNA. Ko te pānga matua o te DNase I ko te whakakino me te wehewehe i te DNA.

Ko te whakamahinga matua o te DNase I kei roto i ngā rangahau koiora ngota. He mea whakamahi noa i roto i ngā whakamātautau maha hei wehe me te pure i te DNA, hei tango i te poke o te DNA ira mai i ngā tauira RNA, me te whakahaere i te DNA. He tino whai hua te DNase I ki te whakaiti i te DNA i roto i ngā matatini pūmua-DNA, e āhei ai ngā kairangahau ki te ako i ngā taunekeneke pūmua-DNA, ki te tango rānei i ngā pūmua e herea ana ki te DNA.

Hei tāpiritanga, he maha ngā whakamahinga o te DNase I i roto i ngā whakamātautau ira DNA, e āwhina ana ki te tautuhi i ngā rohe o te DNA e herea ana e ngā pūmua motuhake. Mā te rongoā i tētahi tauira DNA ki te DNase I, ā, muri iho ka tātari i ngā kongakonga DNA ka puta, ka taea e ngā kairangahau te whakatau i ngā pae here o ngā pūmua here DNA.

Ka whakamahia hoki te DNase I i roto i te tātaritanga chromatin, hei āwhina i te nakunaku o te chromatin (te DNA me ngā pūmua e pā ana) hei ako i tōna hanganga me tōna whakaritenga. Mā te nakunaku i te chromatin ki te DNase I, ka taea e ngā kairangahau te tautuhi i ngā rohe e ngāwari ake ana te uru atu me te tairongo ki te tapahi, ka hoatu he māramatanga ki te whakahaere ira me te hanganga chromatin.

Hei tāpiritanga, he whakamahinga tō te DNase I i roto i ngā tātaritanga hauora, inā koa i roto i te kimi me te ine i te DNA kore-pūtau i roto i te toto e rere ana. Mā te whakamahi i te DNase I hei keri i te DNA kāore e rere ana, ka taea te tātari me te kite i ngā raupapa DNA motuhake e pā ana ki ngā mate, ki ngā hapa ira rānei.