DDT CAS:3483-12-3 Utu Kaihanga

Ko te DL-Dithiothreitol, e mōhiotia ana ko te DTT, he matū whakaiti e whakamahia whānuitia ana i roto i ngā rangahau koiora matū me te koiora ngota. He ngota iti tēnei me te rōpū thiol (kei roto he whanariki) i ia pito.

He maha ngā wā ka whakamahia te DTT hei wawahi i ngā here disulfide i roto i ngā pūmua, ka āwhina i te whakatuwhera, i te whakakore rānei i a rātou. He mea nui tēnei whakaiti i ngā here disulfide i roto i ngā tikanga taiwhanga maha pērā i te purenga pūmua, te electrophoresis gel, me ngā rangahau hanganga pūmua. Ka taea hoki te whakamahi i te DTT hei tiaki i ngā rōpū thiol me te ārai i te waikura i roto i ngā tukanga whakamātautau.

Ko te tikanga ka tāpirihia te DTT ki ngā otinga whakamātautau i roto i ngā kukū iti, ā, ko tōna mahi e whakawhirinaki ana ki te aroaro o te hāora. He mea nui kia āta whakahaerea te DTT nā te mea he tairongo ki te hau, te wera, me te makuku, ka taea te whakaiti i tōna whai huatanga.

Tonohia, Tonoa rānei he Tāpaetanga

Taipitopito Hua

Ngā Tohu Hua

Te Whakamahinga me te Pānga

Te Whakaiti i ngā Here Disulfide: Ko te DTT te mea matua hei wawahi i ngā here disulfide, arā, ngā here covalent i hangaia i waenga i ngā toenga cysteine e rua i roto i ngā pūmua. Mā te whakaiti i ēnei here, ka āwhina te DTT ki te whakakore i te āhua o ngā pūmua, ka taea ai te ako i ō rātou hanganga me ā rātou mahi.

Te Whakatakotoranga Pūmua: Ka āwhina te DTT i te whakatakotoranga tika o te pūmua mā te ārai i te hanganga hē o te here disulfide. Ka whakaitihia e ia ngā here disulfide kore-taketake ka puta i te wā e whakatakotoranga ana te pūmua, ka taea ai e te pūmua te tango i tōna āhua taketake.

Te Mahi a te Whākōkī: Ka taea e te DTT te whakahohe i ētahi whākōkī mā te whakaiti i ngā here disulfide aukati e wātea ana. Hei tāpiri, ka taea e te DTT te aukati i te waikura o ngā toenga cysteine nui, e tika ana pea mō te mahi a te whākōkī.

Te Hanga Paturopi: He mea tāpiri noa te DTT hei whakaiti i ngā here disulfide i te wā e hangaia ana ngā paturopi. Ka āwhina i te aukati i te hanganga o ngā here disulfide hē, ka ārai pea i te here tika o te paturopi.

Te Whakapūmau i ngā Pūmua: Ka taea te whakamahi i te DTT hei whakapūmau i ngā pūmua mā te aukati i tō rātou waikura, i te whakakotahitanga rānei. Ka āwhina i te pupuri i te āhua iti o ngā pūmua i te wā rokiroki me ngā tukanga whakamātautau.

Ngā Āhua Whakaiti i roto i te Koiora Ngota: He maha ngā wā ka whakamahia te DTT i roto i ngā tikanga koiora ngota pēnei i te raupapatanga DNA, te PCR, me te purenga pūmua. Ka taea e ia te āwhina ki te pupuri i te āhua iti o ngā wāhanga matua, kia pai ai ngā hua whakamātautau.

Tauira Hua

Te Tākai Hua:

Ngā Mōhiotanga Tāpiri:

Te hanganga	C4H10O2S2
Whakamātautau	99%
Āhua	Paura mā
Nama CAS	3483-12-3
Te tākai	Iti, nui hoki
Te Ora o te Wāhi	2 tau
Rokiroki	Penapena ki te wāhi hauhautanga, maroke hoki
Tiwhikete	ISO.