BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine tote CAS:6578-06-9

Ko te tote p-toluidine 5-Bromo-4-chloro-3-indolyl phosphate he matū e whakamahia whānuitia ana i roto i te koiora ngota me te rangahau matū koiora. He maha ngā wā e whakamahia ana i roto i ngā whakamātautau immunosorbent enzyme-linked (ELISA) me ngā tikanga tapanga pūmua.

He matū tēnei mō te phosphatase kawakore, he whākōkī e whakamahia whānuitia ana i roto i te koiora ngota hei kimi i te aroaro o ngā ngota ngota motuhake, ngā pūmua rānei mā roto i ngā tauhohenga tae, tauhohenga chemiluminescent rānei.

Ina kitea te whākōkī phosphatase kawakore, ka tapahia e ia ngā rōpū phosphate mai i te tote p-toluidine 5-Bromo-4-chloro-3-indolyl phosphate, ka hua ake he tae kikorangi, he papura rānei. Ka taea te kite me te ine i tēnei huringa tae, ka taea ai te ine i te mahi whākōkī, te aroaro rānei o ngā ngota whainga.

Tonohia, Tonoa rānei he Tāpaetanga

Taipitopito Hua

Ngā Tohu Hua

Te Whakamahinga me te Pānga

Te whakapūtanga o te uruwhenua: Ka whakamahia te BCIP hei kimi me te tiro i ngā pūmua i whakawhitia ki runga i ngā kiriuhi i muri i te hikohiko ira. Ka whakamahia ngā paturopi tuarua kua honoa ki te phosphatase kawakore hei here ki ngā paturopi tuatahi, ā, ko te whakatipuranga i muri mai me te BCIP ka hua ake he putunga kikorangi ki te wāhi o ngā roopu pūmua.

Matū Ārai Mate: Ka whakamahia te BCIP i roto i ngā tikanga tae ārai mate hei kite i te aroaro o ngā pūmua motuhake i roto i ngā kiko. Ka whakamahia ngā paturopi tuarua kua honoa ki te kawakore phosphatase hei here ki ngā paturopi tuatahi, ā, ka puta he tae kikorangi i te aroaro o te whākōkī, e tohu ana i te wāhi o te pūmua i roto i te tauira kiko.

Te whakawhānautanga in situ: Ka whakamahia hoki te BCIP hei kimi i ngā raupapatanga waikawa karihi i roto i ngā kiko, i ngā pūtau rānei kua pūmau. Ka tohua ngā whakamātautau whakawhānautanga ki te alkaline phosphatase, ā, i te tāpiritanga o te BCIP, ka tohuhia te aroaro o te mRNA, te DNA rānei e te whanaketanga o te tae kikorangi.

Te Tākai Hua:

Ngā Mōhiotanga Tāpiri:

Te hanganga	C8H6BrClNO4P.C7H9N
Whakamātautau	99%
Āhua	Paura mā
Nama CAS	6578-06-9
Te tākai	Iti, nui hoki
Te Ora o te Wāhi	2 tau
Rokiroki	Penapena ki te wāhi hauhautanga, maroke hoki
Tiwhikete	ISO.