BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium tote CAS:102185-33-1

Pānga:

He matū kore tae te BCIP ka hurihia hei pūhui kikorangi-papura i runga i te mahi a te alkaline phosphatase. Mā tēnei huringa tae ka taea te kite ā-kanohi me te ine i te mahi a te whākōkī.

Ngā tono:

Ngā whakamātautau whākōkī: Ka whakamahia te BCIP i roto i ngā whakamātautau matū koiora maha hei ine i te mahi a te phosphatase kawakore. Kei roto i tēnei ko te kimi i te mahi phosphatase i roto i ngā lysate pūtau, ngā whākōkī parakore, ngā whakamātautau in vitro rānei.

Matū Ārai Mate: Ka taea te whakamahi i te BCIP hei tiro i ngā paturopi kua tohua ki te phosphatase alkaline i roto i ngā wāhanga kiko, i runga rānei i ngā mata pūtau. He maha ngā wā ka whakamahia tēnei tikanga i roto i ngā tikanga whakatārai mate.

Ngā tikanga whakamōmona: Ka whakamahia te BCIP i roto i te Western blotting, te Southern blotting, me te Northern blotting hei kimi i ngā pūmua motuhake, i ngā waikawa karihi rānei kua tohua ki ngā whakamātautau alkaline phosphatase-conjugated.

Te whakawhānautanga in situ: Ka taea te whakamahi i te BCIP hei whakaatu i ngā tauira whakaaturanga ira i roto i ngā kiko, i ngā pūtau rānei mā roto i ngā tikanga whakawhānautanga in situ.

Ngā whakamātautau tātaritanga: Ka whakamahia te BCIP hei tūāpapa i roto i ngā kete tātaritanga maha hei kimi i te phosphatase kawakore. Ka whakamahia ēnei kete i roto i ngā taiwhanga haumanu hei tātaritanga me te aroturuki i ngā mate.