Avidin CAS:1405-69-2 Utu Kaihanga

Te Whakamaemaetanga Pūmua: Ka taea te pupuri i te Avidin ki runga i tētahi tautoko totoka, pērā i te kapia chromatography, i ngā pirepire aukume rānei, ā, ka whakamahia hei hopu i ngā pūmua biotinylated, i ngā waikawa karihi rānei. E mōhiotia ana tēnei tikanga ko te chromatography affinity avidin, ā, ka taea te whakamaemae i ngā ngota ngota motuhake i roto i tētahi huarahi tino motuhake.

Ngā Pūwero Whakapūmau Koiora: He maha ngā wā ka honoa te Avidin ki ngā whākōkī, ki ngā fluorophores, ki ētahi atu tapanga rānei, ā, ka whakamahia hei matū kimi i roto i ngā momo whakamātautau. Ka taea te kimi i ngā pūwero whakapūmau koiora, pērā i ngā paturopi, i ngā pūwero waikawa karihi rānei, mā te herea ki ngā ngota kimi e hono ana ki te avidin. He tikanga tēnei e whakamahia whānuitia ana i roto i te ELISA, te immunohistochemistry, me ngā whakamātautau whakapūmau waikawa karihi.

Te Tukunga Rongoā Whaiaro: Ka taea te whakamahi i te Avidin hei ngota kawe hei tuku rongoā, hei rongoā rānei ki ngā pūtau, ki ngā kiko rānei e whakaatu ana i te biotin. Mā te hono i te biotin ki ngā rongoā, ki ngā matūriki nano rānei, me te hono atu ki te avidin, ka taea te waihanga i ngā pūnaha tuku rongoā whaiaro.

Ngā Tikanga Koiora Ngongo: He whānuitia te whakamahinga o ngā pūnaha e ahu mai ana i te Avidin i roto i ngā rangahau koiora ngongo. Hei tauira, ko te streptavidin, he rite te kaha here ki te biotin ki te avidin, e whakamahia ana i roto i ngā tikanga pēnei i te raupapatanga DNA, te PCR, me te whakahuihui in situ, e honoa ana ki ngā probes biotinylated, ki ngā primers rānei.

Whakaahua me te Tātaritanga: Ka taea te tāpiri i ngā tohu ki te Avidin mā te whakamahi i ngā isotopes radioactive, ngā tae fluorescent, ngā nanoparticles rānei, ā, he taputapu whai hua tēnei i roto i ngā tikanga whakaahua pērā i te positron emission tomography (PET), te fluorescence microscopy, me te magnetic resonance imaging (MRI). Hei tāpiri, ka whakamahia ngā pūnaha e hangai ana ki te avidin i roto i ngā whakamātautau tātaritanga, pērā i te immunoassays, hei kite i te aroaro o ngā tohu koiora motuhake.