5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

Ko te 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) he matūriki chromogenic e whakamahia whānuitia ana mō te kimi i te mahi beta-glucuronidase (GUS). Ko te GUS he whākōkī e kitea ana i roto i ngā momo rauropi, tae atu ki ngā huakita, ngā tipu, me ngā kararehe whakangote. He maha ngā wā ka whakamahia te X-Gluc i roto i ngā whakamātautau pūrongo GUS me te rangahau koiora ngota.

Ko te whakamahinga matua o X-Gluc kei roto i ngā tikanga tākai histochemical, ka taea ai te whakaatu i te whakaaturanga me te mahi a te whākōkī GUS. Ka taea e tēnei matū te uru ki roto i ngā pūtau, ā, ka waikurahia e te GUS, ka hua ake he putunga kikorangi, he hua kore-rewa rānei. Mā tēnei tākai kikorangi ka taea e ngā kairangahau te tautuhi me te kimi i te mahi a te GUS i roto i ngā pūtau, i ngā kiko, me ngā rauropi katoa.

Ka taea hoki te whakamahi i te X-Gluc i roto i ngā whakamātautau tauanga hei ine i te mahi a te whākōkī GUS. Ka taea te hono i te kaha o te tae kikorangi, te nui rānei o te hua i hangaia ki te taumata o te whakaaturanga GUS, ki tōna mahi whākōkī rānei.

Hei tāpiritanga, kua whakamahia a X-Gluc i roto i ngā rangahau ira tipu hei ako i te whakaaturanga ira, te mahi whakatairanga, me te whakawhiti tipu. Kua whakamahia hoki i roto i ngā pūnaha huakita hei tāruarua me te kimi i ngā pūmua whakakotahitanga GUS.