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ngā hua

Ngā Hua

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide tote cyclohexylammonium CAS:18656-96-7

Ko te tote cyclohexylammonium 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide, e mōhiotia ana ko te X-glucuronide, he matū e whakamahia whānuitia ana i roto i te koiora ngota me ngā whakamātautau enzymology. He matū chromogenic e whakamahia ana hei kimi i te mahi a te whākōkī beta-D-glucuronidase.

Kei roto i te pūhui he rōpū indole e honoa ana ki te wāhanga waikawa glucuronic mā te here glycosidic. Ka whakaratohia e ngā whakakapinga bromo me chloro te pumau me te whakarei ake i te wairewa o te pūhui.

Ina whakatōkia ki tētahi tauira kei roto ko te beta-D-glucuronidase, ka tapahia e te whākōkī te here glycosidic, ka tukuna te wāhanga indole. Ko te hua o tēnei tauhohenga he hanganga kikorangi, he panoni tae rānei, e ngāwari ai te kite i te mahi whākōkī mā te tirohanga, mā te ine-ira rānei.

Nā tōna motuhaketanga mō te beta-D-glucuronidase, e whakamahia whānuitia ana te X-glucuronide i roto i ngā tikanga koiora ngota pēnei i ngā whakamātautau whakawhiti huakita me te rewena, ngā whakamātautau ira pūrongo, me te tae histochemical. He huarahi watea, tairongo hoki tēnei hei kite i te aroaro, i te whakaaturanga rānei o te beta-D-glucuronidase, ā, he maha ngā wā ka whakamahia ngātahi me ētahi atu whakamātautau ira, matū koiora rānei.


Taipitopito Hua

Ngā Tohu Hua

Te Whakamahinga me te Pānga

Ko te pānga o te tote cyclohexylammonium 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-glucuronide) ko tōna kaha ki te whakaputa i tētahi pūhui kikorangi, i tētahi panoni tae rānei i te aroaro o te whākōkī beta-D-glucuronidase. Ka puta tēnei tauhohenga whākōkī ina tapahia te here glycosidic i waenga i te rōpū indole me te wāhanga waikawa glucuronic e te beta-D-glucuronidase.

Ko te whakamahinga o te X-glucuronide e whakamahia ana i roto i te koiora ngota me ngā whakamātautau whākōkī. He mea whakamahi noa hei matū hei kimi i te mahi a te beta-D-glucuronidase. Ko ētahi o ngā whakamahinga motuhake ko:

Ngā whakamātautau ira pūrongo: He maha ngā wā ka whakamahia te X-glucuronide hei matū i roto i ngā whakamātautau ira pūrongo ina whakamahia te beta-D-glucuronidase hei whākōkī pūrongo. Mā te whakakotahi i te rohe whakatairanga, i te raupapa waehere rānei o tētahi ira e pā ana ki te ira beta-D-glucuronidase, ka taea e ngā kairangahau te ine i te whakaaturanga, te mahi rānei o te ira mā te aroturuki i te hanganga o tētahi pūhui kikorangi, te huringa tae rānei ina tāpirihia te X-glucuronide.

Ngā whakamātautau whakawhiti huakita me te rewena: Ka taea te whakamahi i te X-glucuronide hei kite i te whakawhiti angitu o te huakita, o te rewena rānei mā te whakamahi i tētahi ira kei roto te ira beta-D-glucuronidase. Ka taea e ngā pūtau kua whakawhitia e whakaatu ana i te whākōkī te whakawhaiti i te X-glucuronide, ka puta he huringa tae e kitea ana, he putunga rānei.

Te tae histochemical: Ka whakamahia te X-glucuronide i roto i ngā tikanga tae histochemical hei tiro i te aroaro me te tohatoha o te mahi beta-D-glucuronidase i roto i ngā kiko, i ngā pūtau rānei. Mā tēnei tae ka taea te kimi me te tautuhi i ngā pūtau e whakaatu ana i te whākōkī.

Te Tākai Hua:

试剂包装2

Ngā Mōhiotanga Tāpiri:

Te hanganga C26H36BrClN2O7
Whakamātautau 99%
Āhua Paura mā
Nama CAS 18656-96-7
Te tākai Iti, nui hoki
Te Ora o te Wāhi 2 tau
Rokiroki Penapena ki te wāhi hauhautanga, maroke hoki
Tiwhikete ISO.

 


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