4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Pānga: Ko te ONPG he matūriki e whakamahia ana hei kite i te aroaro me te mahi a te whākōkī β-galactosidase. Ina kitea te whākōkī β-galactosidase, ā, ina kaha, ka tapahia e ia te ONPG kia rua ngā hua: te o-nitrophenol me te galactose derivative. Ko te tukunga o te o-nitrophenol ka hua ake he huringa tae kōwhai, ka taea te ine mā te whakamahi i te spectrophotometer.

Te Whakamahinga: He maha ngā whakamahinga o te ONPG i roto i te koiora ngota me te rangahau matū koiora:

Te whakatau i te mahi a te β-galactosidase: E whakamahia whānuitia ana te ONPG hei ine me te ine i te mahi a te whākōkī β-galactosidase. Ka taea te ine i te tere o te hanganga o te o-nitrophenol, e rite tonu ana ki te mahi a te whākōkī, mā te ine inenga.

Te Whakapuakitanga me te Whakahaere ira: He maha ngā wā ka whakamahia te ONPG i roto i ngā whakamātautau e pā ana ki ngā rangahau whakahaere me te whakahaere ira. He maha ngā wā ka whakamahia i roto i ngā whakamātautau pūmua hanumi, pērā i te pūnaha hanumi lacZ e whakamahia whānuitia ana, hei ako i te whakaaturanga o ngā ira i raro i te mana o ngā kaiwhakatairanga motuhake. Mā te mahi beta-galactosidase i inehia mā te ONPG ka kitea te taumata o te whakaaturanga ira.

Te tirotiro i te mahi a te β-galactosidase: Ka taea te whakamahi i te ONPG hei tikanga tirotiro tae i roto i te hangarau DNA recombinant hei tautuhi i te aroaro me te mahi a te ira LacZ, e whakamārama ana i te β-galactosidase. Ka āwhina tēnei tikanga tirotiro ki te tautuhi i ngā ira tārua kei roto te ira e hiahiatia ana.

Ngā rangahau mō te nekenekehanga o te whākōkī: He pai hoki te ONPG mō te ako i te nekenekehanga o te whākōkī β-galactosidase. Mā te ine i te tere o te tauhohenga whākōkī-papa i ngā kukū papa rerekē, ka taea te whakatau i ngā tawhā nekenekehanga pēnei i ngā pūmau Michaelis-Menten (Km) me ngā tere tauhohenga mōrahi (Vmax).