4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Ngā matū whākōkī: Ka taea te whakamahi i te 4NPM hei matū mō ngā momo whākōkī, tae atu ki ngā glycosidase me ngā whākōkī e pā ana. Ka tapahia e ēnei whākōkī te hononga glycosidic i waenga i te mannose me te 4NPM, ka hua ake te tukunga o te wāhanga nitrophenyl. Ka taea te ine i te whānuitanga o te hydrolysis o te matū mā te spectrophotometric mā te aroturuki i te mimiti o te rōpū nitrophenyl kua tukuna i tētahi roangaru motuhake. Mā tēnei ka taea e ngā kairangahau te aromatawai i te mahi whākōkī me te kinetics.

Ngā Whakamātautau mō te pākia o te warowaihā: Mā te whakamahi i te 4NPM hei tūāpapa, ka taea e ngā kairangahau te ako i te mahi a ngā whākōkī e uru ana ki te pākia o te warowaihā, pērā i te alpha-mannosidases. Ka whakawhaitihia e ēnei whākōkī ngā hononga glycosidic i roto i ngā matū kei roto te mannose, ā, ka taea te ine i tā rātou mahi mā te aroturuki i te tukunga o te wāhanga nitrophenyl mai i te 4NPM.

Ngā rangahau glycosylation: Ka taea hoki te whakamahi i te 4NPM i roto i ngā whakamātautau hei tirotiro i ngā whākōkī e whai wāhi ana ki ngā tukanga glycosylation. Ko te glycosylation te tukanga o te tāpiri i ngā ngota huka ki ngā pūmua, ki ētahi atu ngota rānei, ā, he maha ngā whākōkī e whai wāhi ana ki tēnei tukanga. Mā te whakamahi i te 4NPM hei matū tango, ka taea e ngā kairangahau te ine i te whakawhiti o tētahi wāhanga huka ki te 4NPM e ngā whākōkī motuhake e whai wāhi ana ki ngā tauhohenga glycosylation.

Te tirotiro i ngā aukati whākōkī, ngā kaiwhakaoho rānei: Ka taea te whakamahi i te 4NPM i roto i ngā whakamātautau tirotiro tere-rahi hei tautuhi i ngā matū e aukati ana, e whakahohe ana rānei i ngā whākōkī motuhake. Mā te aromatawai i te pānga o ngā matū whakamātautau ki te waikura, ki te whakarerekētanga rānei o te 4NPM e ngā whākōkī ūnga, ka taea e ngā kairangahau te tautuhi i ngā kaihoko rongoā pea, i ngā whakamātautau matū whai hua rānei hei ako i te mahi whākōkī.