3,3′-Diaminobenzidine CAS:91-95-2

Ko te pānga o te 3,3'-Diaminobenzidine (DAB) he whakaputa i te tae parauri ina tauhohe ki ngā whākōkī peroxidase. Mā tēnei huringa tae ka taea te whakaatu i ngā antigens ūnga, ngā biomolecules rānei e pārekareka ana ki ngā whakamātautau tae immunohistochemistry (IHC) me te immunocytochemistry (ICC). He maha ngā wā ka whakamahia te DAB i roto i ngā rangahau hauora me ngā tono tātaritanga hei kimi me te kimi i ngā antigens, i ngā pūmua rānei motuhake i roto i ngā tauira koiora. Mā te tāpiri i te DAB hei papa, me te tauhohenga whai muri me ngā whākōkī peroxidase, ka puta he matū parauri ki te wāhi o ngā antigens ūnga. He tino whai hua te whakamahinga o te tae DAB ki te ako i ngā mate me ngā āhuatanga rerekē. Mā tēnei ka taea e ngā kairangahau me ngā tohunga mate te whakaatu me te tātari i te tohatoha, te nui, me te wāhi o ngā biomolecules motuhake i roto i ngā kiko, i ngā pūtau rānei. Ka āwhina ēnei mōhiotanga ki te mārama ki ngā tikanga mate, ki te ako i ngā tukanga pūtau, me te tautuhi i ngā whāinga tātaritanga, rongoā rānei pea. Hei tāpiri, ka taea te whakamahi i te tae DAB i roto i ngā tikanga ngota rerekē hei tirotiro i ngā taunekeneke pūmua-pūmua, hei tautuhi i ngā tauira whakaaturanga pūmua, hei whakatau i te aroaro o ngā tohu motuhake, me te aromatawai i te whai huatanga o ngā wawaotanga rongoā. Nā tōna tae pono me te pakari, me tōna hototahi ki ngā pūnaha kite me ngā tikanga karuārai rerekē, kua noho te DAB hei papa chromogenic e whakamahia whānuitia ana i roto i ngā whakamātautau immunohistochemistry me te immunocytochemistry.