3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Te kimi i te mahi a te beta-galactosidase: He maha ngā wā ka whakamahia te ONPG hei whakatau i te aroaro me te mahi a te beta-galactosidase i roto i ngā tauira koiora, pērā i ngā ahurea huakita, i ngā lysate pūtau rānei. Ka taea te ine ngāwari i te hanga o te o-nitrophenol, he kōwhai te tae, mā te whakamahi i te spectrophotometer.

Ngā rangahau whakaputa ira: He mea whakamahi noa te ONPG i roto i ngā rangahau koiora ngota hei ako i te whakaputa ira. Mā te whakakotahi i te kaiwhakatairanga o tētahi ira e hiahiatia ana me te ira e whakamārama ana i te beta-galactosidase, ka taea e ngā kairangahau te ine i te mahi a tēnei kaiwhakatairanga mā te tāpiri i te ONPG me te ine i te hua o te whakaputanga o-nitrophenol. Ko tēnei tikanga, e mōhiotia ana ko te whakamātautau kairīpoata beta-galactosidase, e whakarato ana i ngā mōhiohio e pā ana ki te mahi whakawhiti o tētahi ira.

Te tautuhi huakita: Ka whakaputahia e ētahi huakita te beta-galactosidase, ko ētahi kāore. Ka taea te whakamahi i te ONPG me ētahi atu whakamātautau matū koiora hei tautuhi i ngā momo huakita i runga i tō rātou kaha ki te whakawhaiti i te ONPG. He tikanga tēnei e whakamahia whānuitia ana i roto i ngā taiwhanga tātaritanga haumanu me te koiora moroiti.

Te tirotiro i ngā aukati whākōkī, ngā kaiwhakaoho rānei: Ka taea te whakamahi i te ONPG hei tirotiro i ngā matū e whakarerekē ana i te mahi a te beta-galactosidase. Mā te ine i te mahi whākōkī i te aroaro o ngā matū rerekē, ka taea e ngā kairangahau te tautuhi i ngā aukati, ngā kaiwhakaoho rānei ka taea te rangahau anō mō ​​tō rātou kaha rongoā.