3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

Ko te CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) he hopi horoi e whakamahia whānuitia ana i roto i te matū koiora me te koiora ngota. He hopi horoi zwitterionic tēnei, arā, he rōpū utu pai, he rōpū utu kino hoki tōna.

E mōhiotia ana a CHAPS mō tōna kaha ki te whakarewa me te whakau i ngā pūmua kiriuhi, ā, he mea whai hua mō ngā momo mahi pēnei i te tango pūmua, te purenga, me te whakaahua i ngā āhuatanga. Ka whakararuraru i ngā taunekeneke lipid-pūmua, ka taea ai te tango i ngā pūmua kiriuhi i tōna āhua taketake.

He ngawari te CHAPS, he rerekē ki ētahi atu hopi horoi, ā, kāore e whakakore i te āhua o te nuinga o ngā pūmua, he mea pai rawa atu hei pupuri i te hanganga me te mahi a te pūmua i ngā wā whakamātautau. Ka āwhina hoki ki te ārai i te kohikohinga pūmua.

E whakamahia whānuitia ana te CHAPS i roto i ngā tikanga pēnei i te SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), te isoelectric focusing, me te Western blotting. E whakamahia whānuitia ana hoki i roto i ngā rangahau e pā ana ki ngā whākōkī e herea ana ki te membrane, te whakawhiti tohu, me ngā taunekeneke pūmua-lipid.

Tonohia, Tonoa rānei he Tāpaetanga

Taipitopito Hua

Ngā Tohu Hua

Te Whakamahinga me te Pānga

Te Tango Pūmua: He mea noa te whakamahi i te CHAPS hei tango i ngā pūmua kiriuhi mai i ngā tauira koiora. Ka āwhina i te whakarewa i ēnei pūmua me te pupuri i tō rātou hanganga taketake.

Te Whakamaemaetanga Pūmua: E whakamahia ana te CHAPS i roto i ngā tikanga whakamaemaetanga pūmua maha, pērā i te chromatography hononga. Ka taea te tāpiri atu ki ngā arai whakamaemaetanga hei whakarewa me te whakau i ngā pūmua kiriuhi i te wā o te tukanga whakamaemaetanga.

Te Āhuatanga Pūmua: He maha ngā wā ka whakamahia te CHAPS i roto i ngā rangahau e pā ana ki te āhua o ngā pūmua kiriuhi. Ka āwhina i te pupuri i te hanganga me te mahi a te pūmua i roto i ngā tukanga whakamātautau pēnei i ngā whakamātautau ngohe whākōkī, ngā taunekeneke pūmua-pūmua, me ngā tātaritanga matawai.

Ngā Rangahau Pūmua Kiriuhi: He mahi nui tā ngā pūmua kiriuhi i roto i ngā tukanga pūtau maha. He mea whakamahi noa te CHAPS i roto i ngā rangahau e pā ana ki te whakawhiti tohu, te mahi hongere katote, ngā taunekeneke pūmua-lipid, me te whakatiotanga pūmua kiriuhi.

Te Whakapūngao Hiko: E whakamahia ana te CHAPS i roto i ngā tikanga pēnei i te SDS-PAGE me te arotahi isoelectric hei whakarewa i ngā pūmua membrane me te whakahaere i tō rātou wehenga me te tātari.

Te Tākai Hua:

Ngā Mōhiotanga Tāpiri:

Te hanganga	C32H58N2O7S
Whakamātautau	99%
Āhua	Paura tioata mā
Nama CAS	75621-03-3
Te tākai	Iti, nui hoki
Te Ora o te Wāhi	2 tau
Rokiroki	Penapena ki te wāhi hauhautanga, maroke hoki
Tiwhikete	ISO.