2-(tris(hydroxymethyl)methylamino)ethane-1-sulphonic acid CAS:7365-44-8 Whakaahua Matua

Waikawa 2-(tris(hydroxymethyl)methylamino)ethane-1-sulphonic CAS:7365-44-8

Ko te 2-(Tris(hydroxymethyl)methylamino)ethane-1-sulfonic acid, e whakarapopototia ana ko TES, he matū e whakamahia whānuitia ana hei ārai i roto i ngā rangahau koiora me te matū. Hei waikawa sulfonic i ahu mai i te tris(hydroxymethyl)aminomethane (Tris), ka taea e te TES te pupuri i te awhe pH pumau o te 6.8 ki te 8.2. He maha ngā whakamahinga i roto i ngā pāpāho whakatipu pūtau, ngā tauhohenga whākōkī, te electrophoresis pūmua, me te rangahau DNA/RNA. He tino maha ngā whakamahinga o te TES, ā, he mea nui hoki mō te pupuri i ngā āhuatanga tino pai i roto i ngā whakamātautau me ngā tikanga taiwhanga.

Tonohia, Tonoa rānei he Tāpaetanga

Taipitopito Hua

Ngā Tohu Hua

Te Whakamahinga me te Pānga

Ahurea Pūtau: Ka whakamahia te TES i roto i ngā pāpāho ahurea pūtau hei pupuri i te pH pumau, hei whakarite i ngā āhuatanga tino pai mō te tipu me te oranga o ngā pūtau. Ka āwhina i te pumau o te pH ahakoa ngā huringa i puta mai i ngā tukanga pākia, i te tāpiri rānei o ētahi atu matū.

Ngā Tauhohenga Whākōkī: He maha ngā wā ka whakamahia te TES hei āpiha ārai i roto i ngā whakamātautau whākōkī me ngā tauhohenga matū koiora. Ka āwhina ki te pupuri i te pH e hiahiatia ana mō te mahi me te pumau o te whākōkī, kia tika ai, kia taea ai hoki te whakaputa anō i ngā hua.

Te Hikohiko Pūmua: Ka whakamahia te TES i roto i ngā tikanga hikohiko pūmua, pērā i te SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Ka āwhina ki te pupuri i te pH tika i te wā e wehea ana, e tātarihia ana hoki ngā pūmua, kia tika ai te hekenga me te kimi.

Rangahau DNA/RNA: Ka whakamahia te TES i roto i ngā tikanga koiora ngota maha e uru ana te DNA me te RNA. Ka āwhina ki te whakau i te pH i te wā e tangohia ana te waikawa karihi, te purenga, me ngā tikanga whakanui pēnei i te tauhohenga mekameka polymerase (PCR).

Te Tākai Hua:

Ngā Mōhiotanga Tāpiri:

Te hanganga	C6H15NO6S
Whakamātautau	99%
Āhua	Māpaura
Nama CAS	7365-44-8
Te tākai	Iti, nui hoki
Te Ora o te Wāhi	2 tau
Rokiroki	Penapena ki te wāhi hauhautanga, maroke hoki
Tiwhikete	ISO.