-
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid konutai tote CAS:66992-27-6
Ko te N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid konutai tote, e mohiotia ana ko te HEPES konutai tote, he puhui matū e whakamahia nuitia ana hei kaihoko whakamarumaru pH i roto i nga taiwhanga koiora me te matū.Ka awhina i te pupuri i te awhe pH pumau, he pai mo nga tono penei i te ahurea pūtau, te whakamatautau i te enzyme, te rangahau pūmua, te electrophoresis, me te hanga rongoa.Ko te tote konutai HEPES ka whakarite i nga tikanga tino pai mo nga tukanga koiora me te whakanui i te tika me te pono o nga hua whakamatautau.
-
S-Butyrylthiocholine iodide CAS:1866-16-6
Ko te S-Butyrylthiocholine iodide he puhui matū e whakamahia nuitia ana i roto i nga whakamatautau matū koiora me te enzymatic.He taputapu mo te enzyme butyrylcholinesterase (BChE) ka whakamahia hei ine i tana mahi.
Ina whakamarohia te S-Butyrylthiocholine iodide e te BChE, ka puta te thiocholine me te waikawa butyric hei hua.Ko te tukunga o te thiocholine ka taea te ine ma te whakamahi i te spectrophotometric, fluorometric assay ranei, ka taea te ine i te mahi BChE.
Ko te S-Butyrylthiocholine iodide ka whakamahia i roto i nga waahi haumanu me te rangahau hei arotake i te mahi a te BChE i roto i nga tauira penei i te plasma toto, i nga kopa ranei.Ka taea te whakamahi ki te aromatawai i te mahi enzymatic o BChE me tona whai waahi ki roto i nga momo mahi koiora, tae atu ki te tirotiro me te tirotiro i etahi ahuatanga hauora.
-
ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) tote diammonium) CAS:30931-67-0
Ko te Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), e kiia ana ko te ABTS, he taputapu chromogenic e whakamahia ana i roto i nga whakamatautau matū koiora, ina koa i roto i te waahi o te mahi enzymology.He pūhui waihanga e whakamahia ana ki te ine i te mahi o nga momo enzymes, tae atu ki te peroxidases me te oxidases.
Ko te ABTS karekau he tae i roto i tona ahua waikura engari ka huri kikorangi-matomato ina waikurahia e te whākōkī i te aroaro o te hauwai, te hāora rāpoi.Ko tenei huringa tae na te hanganga o te cation tuwhena, e mimiti ana i te marama i roto i te awhiowhio kitea.
Ko te tauhohenga i waenga i te ABTS me te enzyme ka puta he hua tae ka taea te ine ma te spectrophotometrical.Ko te kaha o te tae he orite tika ki te mahi enzymatic, ka taea e nga kairangahau te arotake ine ine kinetics enzyme, inhibition inhibition, or enzyme-substrate interactions.
He maha nga momo tono a ABTS i roto i nga momo waahi, tae atu ki nga whakamatautau haumanu, rangahau rongoa, me te putaiao kai.He tino tairongo me te tuku i te awhe hihiko, na te mea he mea rongonui mo te maha o nga whakamatautau koiora.
-
4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
Ko te 4-Nitrophenyl α-D-maltohexaoside he pūhui no te akomanga o nga hononga α-glycosidic.He huanga mai i te maltose, he disaccharide e rua nga waeine huka.I roto i tenei pūhui, ka whakakapihia te roopu hydroxyl o te wae huka tuatahi ki te wahanga nitrophenyl.
Ka whakamahia tenei puhui hei tïpako i roto i nga whakamatautau enzymatic ki te ako i nga mahi o nga momo enzymes, ina koa ko nga mea e uru ana ki te warowaihā.Ka taea e te roopu nitrophenyl te rapu ngawari me te ine i nga tauhohenga enzymatic ma te ine i te whakaurunga, i te rere ranei o te hua kua mawehea.
-
PIPES CAS:5625-37-6 Utu Kaihanga
Ko te PIPES (piperazine-1,4-bisethanesulfonic acid) he puhui whakaahuru zwitterionic e whakamahia nuitia ana i roto i te rangahau koiora me te matū koiora.He putunga pH whai hua me te kaha nui mo te pupuri i nga tikanga pH pumau i roto i te awhe pH o 6.1 ki te 7.5.He iti noa te wawaotanga o te PIPES ki te koiora me te pai mo nga whakamatautau e whakawhirinaki ana ki te pāmahana.He maha nga wa e whakamahia ana i roto i nga tikanga electrophoresis gel me te hanga rongoa hei kaihoko whakapumau.I roto i te katoa, ko te PIPES he puhui whai kiko me te whakamahi whanui i roto i nga momo waahi whakamatautau.
-
3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
Ko te 3,3′,5,5′-Tetramethylbenzidine, e mohiotia ana ko TMB, he pūhui matū e whakamahia nuitia ana hei tïpako chromogenic i roto i nga whakamatautau immunosorbent e hono ana i te enzyme (ELISA) me etahi atu whakamatautau koiora.He maha nga wa e whakamahia ana ki te kimi me te ine i te noho mai o nga enzyme penei i te horseradish peroxidase (HRP) i roto i nga momo tauira koiora.Ka huri te tae o TMB mai i te kore tae ki te puru i te aroaro o enei whaamua.Whai muri, ka taea te aukati i te tauhohenga ma te taapiri i te waikawa hei huri i te tae kahurangi ki te tae kowhai whakamutunga.Ko te kaha o te tae kōwhai he rite ki te nui o te whākōkī e noho ana, ka taea te ine.
.
-
APS-5 CAS:193884-53-6 Utu Kaihanga
Ko te (4-Chlorophenyl)thio-methanol 1-(dihydrogen phosphate) te tote disodium (1:2) he puhui matū no te karaehe o te acridine derivatives.Kei roto he punaha mowhiti 10-methylacridine me te roopu thioether e piri ana ki te tuunga 4-chlorophenyl.Kei roto ano i te puhui he roopu methanol me nga roopu phosphate e rua kua whakakorehia e te konutai katote.
-
5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1
Ko te 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) he pūhui matū e whakamahia nuitia ana i roto i te koiora rāpoi me te tono matū koiora.He tïpako chromogenic mo nga enzyme phosphatase alkaline.
He maha nga wa e whakamahia ana te BCIP me te nitroblue tetrazolium (NBT) hei tïpako kia kitea te ngohe phosphatase alkaline.Ina whakakorehia te BCIP e te alkaline phosphatase, ka puta he rerenga kikorangi, ka taea te kite i te noho o te enzyme, te mahi ranei.
He tino whaihua tenei puhui ki nga tono penei i te immunohistochemistry, in situ hybridization, me te enzyme-linked immunosorbent assays (ELISAs) ki te kite i te noho, i te waahi ranei o nga koiora motuhake, waikawa karihi ranei.Ko te rerenga kikorangi i hangaia e te BCIP he tohu ka kitea hei awhina i te tautuhi me te tātaritanga o nga ngota ngota i whaaia i roto i nga tauira whakamatautau.
-
Te tote konutai CAS:139-41-3 Utu Kaihanga
Ko te tote konutai N,N-Bis(2-hydroxyethyl)glycine he pūhui matū e whakamahia ana hei parepare i roto i nga momo tono matū-koiora me te koiora.Ka awhina ia ki te pupuri i te taumata pH pumau i roto i nga ahuatanga whakamatautau, ka whai hua ki nga rangahau whaariki, rangahau pūmua, ahurea pūtau, me nga tikanga whakakore i te Tai Hauauru.
-
4-Aminophthalhydrazide AMPPD CAS:3682-14-2
Ko te 4-Aminophthalhydrazide, e mohiotia ana ko 4-APhH, he pūhui matū me te tātai rāpoi C8H8N2O.No te karaehe o nga pūhui hydrazide ka ahu mai i te waikawa phthalic.
.
-
N-Acetyl-L-cysteine CAS:616-91-1
Ko te N-Acetyl-L-cysteine (NAC) he ahua whakarereke o te waikawa amino cysteine.Ka whakaratohia he puna o te cysteine ka taea te huri ki te tripeptide glutathione, he antioxidant kaha i roto i te tinana.E mohiotia ana te NAC mo ona taonga antioxidant me te mucolytic, e whai hua ana ki nga momo tono hauora.
Hei antioxidant, ka awhina a NAC ki te tiaki i nga pūtau mai i nga kino i puta mai i nga rauropi kore utu, nga momo hāora tauhohenga, me te paitini.Ka tautoko hoki i te whakahiatotanga o te glutathione, e whai waahi nui ana ki nga tikanga whakaweto o te tinana me te pupuri i te punaha aukati hauora.
Kua rangahauhia te NAC mo ona painga pea mo te hauora manawa, otira mo nga tangata whai mate penei i te bronchitis mau tonu, COPD, me te cystic fibrosis.I te nuinga o te wa ka whakamahia hei expectorant hei awhina i te angiangi me te wetewete i te hūpē, kia māmā ake te whakawātea i ngā huarahi hau.
I tua atu, kua oati te NAC ki te tautoko i te hauora ate ma te awhina ki te tango i nga mea paitini, penei i te acetaminophen, he rongoa mamae noa.Ka whai hua whakamarumaru ano pea ki te kino o te ate na te inu waipiro.
I tua atu i ona taonga antioxidant me te tautoko manawa, kua tirotirohia te NAC mo ona painga pea mo te hauora hinengaro.Ko etahi rangahau e kii ana tera pea ka pa ki nga raruraru o te ngakau, penei i te pouri me te mate whakapouri (OCD).
-
Acetylthiocholine iodide CAS:1866-15-5
Ko te Acetylthiocholine iodide he pūhui matū e whakamahia nuitia ana hei tïpako i roto i te whakamätau i te whäkau hei ine i te ngohe o te acetylcholinesterase (AChE).Ko te AChE he whākōkī e whakamaarama ana i te neurotransmitter acetylcholine, he mahi nui hei whakamutu i te tuku tohu i waenga i nga pūtau nerve.
I te wa e mahihia ana te acetylthiocholine iodide e AChE, ka tangohia te roopu acetyl, ka puta te hanganga o nga katote thiocholine me te acetate.Ka tauhohe a Thiocholine me te reagent kore tae ko DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) ki te whakaputa i tetahi puhui tae kowhai ko 5-thio-2-nitrobenzoate, ka taea te ine ma te spectrophotometrical.Ko te tere o te whakawhanaketanga tae he rite tonu ki te mahi a AChE i roto i te tauira.
