2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Te whākōkī: Ko te ONPG te mea e whakamahia whānuitia ana hei whākōkī mō te beta-galactosidase, he whākōkī e whakakōkī ana i te ONPG hei whakaputa i tētahi matū kōwhai (o-nitrophenol) ka taea te kite ngāwari mā te ine i te mahi a te beta-galactosidase, ā, ka waiho te ONPG hei taputapu whai hua i roto i ngā rangahau whākōkī.

Ngā whakamātautau koiora ngota: Ka whakamahia te ONPG hei tūāpapa i roto i ngā whakamātautau koiora ngota, inā koa i roto i ngā whakamātautau ira pūrongo beta-galactosidase. I roto i ēnei whakamātautau, ka whakamahia he tūāpapa e hangai ana ki te ONPG hei ine i te mahi a te ira pūrongo, e whakahaerehia ana e tētahi raupapa whakatairanga motuhake. Ko te mahi beta-galactosidase, e tohuhia ana e te huringa tae i puta i te hydrolysis ONPG, ka whakarato i ngā mōhiohio whai hua mō te mahi whakatairanga.

Tātaritanga whakaaturanga ira: Ka whakamahia hoki te ONPG i roto i te tātaritanga o te whakaaturanga ira. Mā te hono i te raupapa whakatairanga e hiahiatia ana ki te ira beta-galactosidase, ka taea e ngā kairangahau te ine i te mahi beta-galactosidase mā te whakamahi i te ONPG hei papa. Ko te taumata o te mahi beta-galactosidase e whakaata ana i te kaha me te mahi a te kaiwhakatairanga, e taea ai te aromatawai i ngā taumata whakaaturanga ira.

Ngā tono tātaritanga: Ka taea te whakamahi i te ONPG i roto i ngā tono tātaritanga, inā koa i roto i te kimi i ngā moroiti mate. He maha ngā huakita, pērā i te Escherichia coli me ētahi momo Shigella me Salmonella, ka whakaputa i te beta-galactosidase ka taea te tapahi i te ONPG. Ka puta he panoni tae kitea i tēnei tauhohenga hydrolysis, ka taea te whakamahi hei whakatau i te aroaro o ēnei huakita i roto i ngā tauira haumanu.