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2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Ko te 2-Nitrophenyl-beta-D-glucopyranoside he pūhui matū kei roto he ngota ngota glucopyranoside e piri ana ki te roopu nitrophenyl.Ka whakamahia tonu hei tïpako i roto i nga whakamatautau enzymatic ki te kimi me te ine i te ngohe o nga enzyme penei i te beta-glucosidase.Ka taea te wehe i te roopu nitrophenyl e te enzyme, ka puta mai he hua kowhai-tae ka taea te ine ma te spectrophotometrical.He tino whai hua tenei puhui ki te ako i nga kinetika o te whaariki me te tirotiro teitei o te aukati i te whakakorikori, i nga kaiwhakahohe ranei.Ka whakamahia ano hoki i roto i te rangahau matū koiora mo te tirotiro i te paopaotanga warowaiha me te mea he tïpako motuhake-hononga- glycosidic.


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Taupānga me te Pānga

Tïpako Enzyme: I te nuinga o te wa ka whakamahia te ONPG hei tïpako mo te beta-galactosidase, he enzyme e whakawaiwai ana i te ONPG ki te whakaputa i te puhui tae-kowhai (o-nitrophenol) ka ngawari te kitea ma te whai kiko.Ka taea te whakamahi i tenei tauhohenga enzymatic ki te ine i te ngohe o te beta-galactosidase, ka waiho te ONPG hei taputapu nui i roto i nga rangahau enzymology.

Nga whakamatautau koiora rapoi: Ka whakamahia te ONPG hei tïpako i roto i nga momo whakamatautau koiora rapoi, ina koa i roto i nga whakamatautau ira kairipoata beta-galactosidase.I roto i enei whakamatautau, ka whakamahia he taputapu ONPG hei ine i te mahi o te ira kairipoata, he mea whakarite e tetahi raupapa whakaihiihi o te paanga.Ko te ngohe beta-galactosidase, e tohuhia ana e te huringa tae i puta i te ONPG hydrolysis, e whakarato ana i nga korero nui mo te mahi whakatairanga.

Te tātari ira ira: Ka whakamahia ano te ONPG i roto i te tātaritanga o te whakaaturanga ira.Ma te hono i te raupapa kaiwhakatairanga o te paanga ki te ira beta-galactosidase, ka taea e nga kairangahau te ine i te mahi beta-galactosidase ma te whakamahi i te ONPG hei taputapu.Ko te taumata o te mahi beta-galactosidase e whakaata ana i te kaha me te ngohe o te kaiwhakatairanga, e taea ai te aromatawai i nga taumata whakaaturanga ira.

Nga tono tātari: Ka taea te whakamahi i te ONPG i roto i nga tono tātaritanga, ina koa ki te kitea nga microorganisms pathogenic.Ko nga momo huakita, penei i te Escherichia coli me etahi momo Shigella me Salmonella, ka whakaputa i te beta-galactosidase ka pakaru te ONPG.Ko tenei tauhohenga waiwai ka puta he huringa tae ka kitea, ka taea te whakamahi hei whakatau i te noho o enei huakita i roto i nga tauira haumanu.

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Nga korero taapiri:

Te tito C12H15NO8
Te whakamatautau 99%
Te ahua Te paura kowhai marama
CAS No. 2816-24-2
Te takai Iti me te rahi
Te Ora Papa 2 tau
Rokiroki Rokiroki ki te waahi makariri me te maroke
Tiwhikete ISO.

 


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